Enzyme Electrode

• US 20150129425A1
• Filed: 06/25/2013
• Published: 05/14/2015
• Est. Priority Date: 06/25/2012
• Status: Active Application

• Current Assignee: BioEngineering Laboratories, LLC
• Original Assignee: BioEngineering Laboratories, LLC
• Inventors: Tsukada, Masashi, Tajima, Mayumi
• Application Number:
  US14/410,704
• Publication Number:
  US 20150129425A1
• Time in Patent Office: Days
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875

Abstract

An enzyme electrode includes an electrode, and a detection layer that is in contact with the electrode and includes an oxidoreductase, a water-soluble conductive polymer, and conductive particles, electrons being transferred between the enzyme and the electrode by direct electron transfer in the detection layer.

Claims

1. An enzyme electrode, comprising:

an electrode; and

a detection layer that is in contact with the electrode and comprises an oxidoreductase, a water-soluble conductive polymer, and conductive particles,

wherein electrons are transferred between the enzyme and the electrode by direct electron transfer in the detection layer.

2. The enzyme electrode according to claim 1, wherein the water-soluble conductive polymer is polyaniline sulfonic acid.

3. The enzyme electrode according to claim 1, wherein the oxidoreductase has a subunit containing cytochrome.

4. The enzyme electrode according to claim 1, wherein the oxidoreductase has a domain containing cytochrome.

5. The enzyme electrode according to claim 1, wherein the oxidoreductase is an oligomeric enzyme including at least a catalytic subunit and a subunit containing cytochrome.

6. The enzyme electrode according to claim 1, wherein the oxidoreductase is an enzyme including at least a catalytic domain and a domain containing cytochrome.

7. The enzyme electrode according to claim 5, wherein the catalytic subunit includes at least any one of pyrroloquinoline quinone and flavin adenine dinucleotide.

8. The enzyme electrode according to claim 6, wherein the catalytic domain includes at least any one of pyrroloquinoline quinone and flavin adenine dinucleotide.

9. The enzyme electrode according to claim 1, wherein the conductive particles comprise carbon.

10. The enzyme electrode according to claim 2, wherein the concentration of the polyaniline sulfonic acid is 0.01 to 2%.

11. The enzyme electrode according to claim 2, wherein a functional group of the polyaniline sulfonic acid is a hydroxy group or a sulfo group.

12. The enzyme electrode according to claim 1, wherein the enzyme electrode has an improved heat resistance.

13. A biosensor, comprising:

an enzyme electrode, the enzyme electrode including;

an electrode; and

a detection layer that is in contact with the electrode and includes an oxidoreductase, a water-soluble conductive polymer, and conductive particles,

wherein electrons are transferred between the enzyme and the electrode by direct electron transfer in the detection layer.

14. An electronic apparatus comprising the biosensor according to claim 13.

15. An apparatus, comprising:

an enzyme electrode; and

a feed section configured to supply, to a load, a current generated by an enzyme reaction at the enzyme electrode, the enzyme electrode including an electrode and a detection layer that is in contact with the electrode and comprises an oxidoreductase, a water-soluble conductive polymer, and conductive particles, wherein electrons are transferred between the enzyme and the electrode by direct electron transfer in the detection layer.

16. An electronic apparatus comprising the device according to claim 15.

17. A method for producing an enzyme electrode, comprising:

forming a detection layer on an electrode, the detection layer comprising an oxidoreductase, a water-soluble conductive polymer, and conductive particles,

wherein electrons are transferred between the enzyme and the electrode by direct electron transfer.

18. A reagent having improved heat resistance, comprising:

an oxidoreductase;

a water-soluble conductive polymer; and

conductive particles.