FLUORESCENCE DETECTOR FOR MICROFLUIDIC DIAGNOSTIC SYSTEM
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Abstract
The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
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Citations
38 Claims
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1-16. -16. (canceled)
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17. A diagnostic apparatus, comprising:
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a microfluidic cartridge comprising a planar surface having a first microfluidic channel with a first amplification chamber, a second microfluidic channel with a second amplification chamber, a third microfluidic channel with a third amplification chamber, and fourth microfluidic channel with a fourth amplification chamber;
the microfluidic channels aligned adjacent to each other at a first end of the microfluidic cartridge, the first and the second amplification chambers aligned along a first axis, the third and the fourth amplification chambers aligned along a second axis, wherein the first axis is substantially parallel to the second axis, and each amplification chamber configured to contain one or more polynucleotides;a scanning read head comprising a first and second optical detection blocks, each optical detection block having a first detection unit and a second detection unit aligned along a detection axis, the first detection unit configured to measure a particular frequency of light from a particular location, the second detection unit configured to measure a particular frequency of light from a particular location, wherein the detection axis of the first optical detection block and the first axis are coplanar in a first plane perpendicular to the planar surface of the microfluidic cartridge, wherein the scanning read head is configured to travel in a direction transverse to the detection axis to a position wherein the detection axis of the first optical detection block and the second axis are coplanar in a second plane perpendicular to the planar surface of the microfluidic cartridge; wherein each detection unit comprises a light source configured to transmit a beam of focused light on to a particular region of the microfluidic cartridge; and a light detector configured to receive light that is emitted from the particular region of the microfluidic cartridge. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A diagnostic apparatus, comprising:
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a planar surface of a microfluidic cartridge comprising a first microfluidic channel with a first amplification chamber, a second microfluidic channel with a second amplification chamber, a third microfluidic channel with a third amplification chamber, the microfluidic channels aligned adjacent to each other at a first end of the microfluidic cartridge, wherein the first and the second amplification chambers are aligned along a first axis, wherein the third amplification chamber is aligned along a second axis parallel to the first axis, each amplification chamber configured to contain one or more polynucleotides; an optical detection block having a first detection unit and second detection unit aligned along a detection axis, wherein the optical detection block is configured to travel in a direction transverse to the detection axis from a first position wherein the detection axis and the first axis are coplanar in a first plane perpendicular to the planar surface to a second position wherein the detection axis and the second axis are coplanar in a second plane perpendicular to the planar surface, wherein each detection unit comprises a light source configured to transmit a beam of focused light on to a particular region of the microfluidic cartridge; and a light detector configured to receive light that is emitted from the particular region of the microfluidic cartridge. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35)
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36. A diagnostic apparatus, comprising:
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a microfluidic cartridge having a planar surface comprising a plurality of separate microfluidic channels, each channel comprising an amplification chamber, each channel aligned adjacent to each other at a first end of the microfluidic cartridge;
wherein the amplification chambers of a first and a second adjacent microfluidic channel are aligned co-axially along a first axis and wherein the amplification chambers of a third and a fourth adjacent microfluidic channel are aligned co-axially along a second axis, each amplification chamber configured to contain one or more polynucleotides;a first detection unit aligned along a detection axis, the first detection unit comprising a first light source configured to transmit a beam of focused light on to any of the amplification chambers and a first light detector configured to receive light that is emitted from any of the amplification chambers; and a second detection unit aligned along the detection axis, the second detection unit comprising a second light source configured to transmit a beam of focused light on to any of the amplification chambers and a second light detector configured to receive light that is emitted from any of the amplification chambers; wherein in a first position, the detection axis and the first axis lie on a first plane perpendicular to the planar surface of the microfluidic cartridge, and wherein in a second position the detection axis and the second axis lie on a second plane perpendicular to the planar surface of the microfluidic cartridge. - View Dependent Claims (37, 38)
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Specification