DROPLET-BASED SURFACE MODIFICATION AND WASHING
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Abstract
The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
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Citations
64 Claims
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1-23. -23. (canceled)
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24. A droplet microactuator, comprising:
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(a) a first substrate and a second substrate, the second substrate arranged relative to the first substrate and spaced from the surface of the first substrate by a distance sufficient to define a space between the first substrate, wherein the space comprises a filler fluid; (b) electrodes arranged on one or both of the first substrate and second substrate and configured for manipulating a droplet on a surface thereof; (c) one or more reservoirs disposed at one of the first substrate and second substrate; and (d) one or more input ports disposed at one of the first substrate and second substrate. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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49. A method of dispensing a droplet into an interior volume of a droplet microactuator, the method comprising:
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(a) providing a droplet microactuator, comprising; (i) a first substrate and a second substrate, the second substrate arranged relative to the first substrate and spaced from the surface of the first substrate by a distance sufficient to define a space between the first substrate, wherein the space comprises a filler fluid; (ii) electrodes arranged on one or both of the first substrate and second substrate and configured for manipulating a droplet on a surface thereof; (iii) one or more reservoirs at one of the first substrate and second substrate; and (iv) one or more input ports disposed at one of the first substrate and second substrate; (b) providing a quantity of a fluid sample to one of the one or more reservoirs; and (c) dispensing a droplet from the provided fluid sample into the interior volume of the droplet microactuator. - View Dependent Claims (50, 51, 52, 53, 54, 55)
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56. A method of delivering a droplet to a reservoir, the method comprising:
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(a) providing a droplet microactuator, comprising; (i) a first substrate and a second substrate, the second substrate arranged relative to the first substrate and spaced from the surface of the first substrate by a distance sufficient to define a space between the first substrate, wherein the space comprises a filler fluid; (ii) electrodes arranged on one or both of the first substrate and second substrate and configured for manipulating a droplet on a surface thereof; (iii) one or more reservoirs at one of the first substrate and second substrate; (iv) one or more input ports disposed in at least one of the first substrate and second substrate; and wherein, the droplet is situated in the space between the first substrate and second substrate; (b) transporting the droplet along a path or network of the electrodes; and (c) releasing the droplet at an appropriate point along the path or network of the electrodes and allowing the droplet to float or sink, as applicable, to a target destination. - View Dependent Claims (57, 58, 59, 60, 61, 62, 64)
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63. A method of conducting a biochemical reaction, the method comprising:
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(a) providing a droplet microactuator, comprising; (i) a first substrate and a second substrate, the second substrate arranged relative to the first substrate and spaced from the surface of the first substrate by a distance sufficient to define a space between the first substrate, wherein the space comprises a filler fluid; (ii) electrodes arranged on one or both of the first substrate and second substrate and configured for manipulating a droplet on a surface thereof; (iii) one or more reservoirs at one of the first substrate and second substrate, the one or more reservoirs comprising at least one waste reservoir; (iv) one or more input ports disposed in at least one of the first substrate and second substrate; and wherein, the droplet is situated in the space between the first substrate and second substrate; (b) conducting a biochemical reaction, wherein the biochemical reaction produces a waste product; and (c) transporting the waste product along a path or network of the electrodes to the waste reservoir.
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Specification