METHODS OF MAKING QUINOA LEACHATES AND USES THEREOF

• US 20150157644A1
• Filed: 12/05/2014
• Published: 06/11/2015
• Est. Priority Date: 12/06/2013
• Status: Active Grant

• Current Assignee: Rutgers University
• Original Assignee: Rutgers University
• Inventors: Raskin, Ilya, Graf, Brittany
• Granted Patent: US 10,441,594 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A61K 2236/00 Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
• CPC Class Codes: A61K 31/575 substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
• CPC Class Codes: A61K 36/21 Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth

Abstract

The present application is directed to materials and method for producing leachates from a plant of the Amaranthaceae family.

Claims

1. A method of producing a leachate from a plant seed of the Amaranthaceae family comprising:

contacting a seed from a plant of the Amaranthaceae family with a leaching fluid to produce a leaching mixture; and

separating the seed from the leaching mixture, thereby producing a leachate from the plant seed of the Amaranthaceae family.

2. The method of claim 1, wherein the plant seed is a Chenopodium quinoa plant seed.

3. The method of claim 1, wherein the leaching fluid is selected from the group consisting of water, n-butanol, isopropanol, n-propanol, ethanol, methanol, ethylacetate, nitromethane and formic acid.

4. The method of claim 1, wherein the leaching fluid comprises 60-80% ethanol.

5. The method of claim 4, wherein the leaching fluid comprises 70% ethanol.

6. The method of claim 1, wherein the seed is intact.

7. The method of claim 1, wherein the outer layer of the intact seeds from the plant is removed prior to the contacting step.

8. The method of claim 1, wherein the contacting step is performed at a temperature of less than 80°

C.

9. The method of claim 1, wherein the contacting step is performed at room temperature.

10. The method of claim 1, wherein the separating step is performed using a method selected from the group consisting of filtration, sedimentation, centrifugation, evaporation, reduced-pressure distillation, precipitation, lyophilization and adsorption.

11. The method of claim 1, wherein the leachate comprises from about 1 μ

g to about 400 μ

g 20-hydroxyecdysone (20-HE) per gram of seed.

12. The method of claim 1, further comprising drying the leachate.

13. The method of claim 12, wherein the drying is performed by one or more methods selected from the group consisting air drying, spray drying, speed vacuum, rotoevaporation, and lyophilization.

14. The method of claim 1, further comprising purifying the leachate.

15. A leachate produced by the method of claim 1.

16. The leachate of claim 15, wherein the leachate comprises from about 1 μ

g to about 400 μ

g 20-hydroxyecdysone (20-HE) per gram of seed.

17. The leachate of claim 15, wherein the leachate comprises at least 0.5 mg 20-hydroxyecdysone (20-HE) per gram of leachate.

18. The leachate of claim 15, wherein the leachate comprises at least 0.5 mg total polyphenol content per gram of leachate.

19. A leachate produced by the method of claim 12.

20. A leachate produced by the method of claim 14.