PRODUCTION OF ENZYMES FOR LIGNO-CELLULOSIC BIOMASS
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Abstract
A process produces at least a first enzyme from a host cell, wherein the first enzyme is capable of hydrolyzing a first pre-treated ligno-cellulosic biomass. The process comprises the step of cultivating the host cell to produce at least the first enzyme for a cultivation time, wherein the cultivation of the host cell occurs in a sugar depleted cultivation environment comprising the host cell, water and a solid composition comprising a complex sugar of the solid composition and a lignin of the solid composition. In such a process, the solid composition is obtained from a second pre-treated ligno-cellulosic biomass, comprising a complex sugar of the second pre-treated ligno-cellulosic biomass and a lignin of the second pre-treated ligno-cellulosic biomass; and the ratio of the total amount of the complex sugars of the solid composition to the total amount of the lignin of the solid composition is greater than zero and less than the ratio of the total amount of the complex sugars of the second pre-treated ligno-cellulosic biomass to the total amount of the lignin of the second pre-treated ligno-cellulosic biomass.
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Citations
38 Claims
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1-19. -19. (canceled)
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20. A process which produces at least a first enzyme from a host cell, wherein the first enzyme is capable of hydrolyzing a first pre-treated ligno-cellulosic biomass,
said process comprising the step of cultivating the host cell to produce at least the first enzyme for a cultivation time, wherein the cultivation of the host cell occurs in a sugar depleted cultivation environment comprising the host cell, water and a solid composition, said solid composition comprising a complex sugar of the solid composition and a lignin of the solid composition, wherein a) the solid composition is obtained from a second pre-treated ligno-cellulosic biomass, comprising a complex sugar of the second pre-treated ligno-cellulosic biomass and a lignin of the second pre-treated ligno-cellulosic biomass; - and
b) the ratio of the total amount of the complex sugars to the total amount of the lignin in the solid composition is less than the ratio of the total amount of the complex sugars to the total amount of the lignin in the second pre-treated ligno-cellulosic biomass. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification