DRY LAMINATED PHOTOLUMINESCENT PROBE AND METHOD OF MANUFACTURE AND USE

• US 20150177154A1
• Filed: 09/06/2011
• Published: 06/25/2015
• Est. Priority Date: 09/06/2011
• Status: Abandoned Application

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Papkovsky, Dmitri Boris
• Application Number:
  US14/370,865
• Publication Number:
  US 20150177154A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/75 Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated systems in which material is burnt in a flame or plasma G01N21/72, G01N21/73
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence

Abstract

Dry laminated photoluminescent probe (10) and methods of manufacture and use. The probe (10) includes a support layer (30) with a plurality of separate and independent optically active particles (20) dry laminated onto a first major surface (30a) of the support layer (30) forming a sensing area (15) on the support layer (30). The optically active particles (20) are preferably laminated onto the support layer (30) via a layer of pressure sensitive adhesive (40).

Claims

1. A remotely interrogatable optochemical probe which produces a specific measurable optical response to a target analyte from which target analyte can be reliably quantified, the probe comprising:

(a) a support layer having a first major surface,

(b) a first layer of pressure sensitive adhesive coated onto the first major surface of the support layer, and

(c) a plurality of separate and independent optically active particles sensitive to a target analyte, dry laminated and pattern deposited onto the first major surface of the support layer via the first layer of pressure sensitive adhesive coated onto the first major surface of the support layer, so as the form at least one discrete sensing area on the first major surface of the support layer with areas of pressure sensitive adhesive still exposed.

2. The probe of claim 1 further comprising a plurality of separate and independent diluent particles interspersed with and dry laminated onto the first major surface of the support layer along with the optically active particles.

4. The probe of claim 1 further comprising a protective layer covering at least the sensing area.

5. The probe of claim 1 wherein the support law has a second major surface opposite the first major surface, and the probe further comprises a second layer of pressure sensitive adhesive coated onto the second major surface of the support layer.

6. The probe of claim 5 further comprising a release liner covering the second layer of pressure sensitive adhesive.

8. The probe of claim 4 wherein the optically active particles are sensitive to concentration of oxygen in communication with the particles and the protective layer is permeable to oxygen.

11. The probe of claim 1 wherein the support layer is highly permeable to oxygen.

13. The probe of claim 1 wherein the first major surface of the support layer scatters light.

14. The probe of claim 1 wherein the adhesive forms a continuous coating over the entire first major surface of the support layer.

18. The probe of claim 1 further comprising a protective layer covering at least the sensing area and a portion of the exposed adhesive area, whereby the protective layer is laminated to the support layer via the first layer of pressure sensitive adhesive.

20. The probe of claim 1 wherein the sensing area is a single discrete area of between 1 and 100 mm².

22. The probe of claim 1 wherein the sensing area is a single discrete area of between 4 and 30 mm².

24. The probe of claim 1 wherein the optically active particles are particles of a target-analyte permeable polymer impregnated with a target-analyte quenchable photoluminescent material.

25. The probe of claim 24 wherein the target-analyte quenchable photoluminescent material is a target-analyte quenchable long-decay fluorescent or phosphorescent indicator dye.

29. The probe of claim 25 wherein the target-analyte permeable polymer is an oxygen permeable polymer selected from polystyrene and cross-linked poly(styrene-divinylbenzene).

31. The probe of claim 1 wherein the average volume based particle size of the optically active particles is 1 to 200 micrometers.

34. The probe of claim 8 wherein the oxygen permeable protective layer covers at least the sensing area, and is selected from a film of polyethylene, polypropylene, silicon, fluorinated polyolefin and polyvinylchloride

51. A method of monitoring changes in analyte concentration in an enclosed environment, comprising the steps of:

(a) placing a probe is accordance with claim 1 into a chamber,

(b) sealing the probe-containing chamber,

(c) periodically interrogating the probe within the chamber with an interrogation device wherein interrogations measure changes in the probe reflective of changes in analyte concentration within the chamber.

52. The method of claim 51 wherein a test sample is placed into the chamber prior to sealing the chamber whereby changes in analyte concentration within the chamber are attributable to microbial respiration and/or decompositions of the sample

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37. The probe of date 24 wherein (i) the target-analyte quenchable photoluminescent material is excited by light at an excitation wavelength and emits light at an emission wavelength, and (ii) both the substrate and pressure sensitive adhesive transmit light at the excitation and emission wavelengths.

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