METHODS AND COMPOSITIONS FOR GENERATION OF GERMLINE HUMAN ANTIBODY GENES
First Claim
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1. A method for producing a polynucleotide encoding a human germline antibody V-region, comprising the steps of:
- (a) obtaining a V minigene or a J minigene; and
(b) joining the V minigene with at least one J minigene, or joining the J minigene with a V minigene, wherein the J minigene is located at the 3′
end of the V minigene.
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Abstract
The present invention relates to a method for in vitro producing polynucleotides encoding human germline antibody V-regions. Also disclosed is a library of human germline antibody V-region genes.
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Citations
25 Claims
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1. A method for producing a polynucleotide encoding a human germline antibody V-region, comprising the steps of:
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(a) obtaining a V minigene or a J minigene; and (b) joining the V minigene with at least one J minigene, or joining the J minigene with a V minigene, wherein the J minigene is located at the 3′
end of the V minigene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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- 15. A library comprising member polynucleotides encoding exogenously rearranged human germline antibody V-regions.
Specification