IMAGE FORMING METHOD AND IMAGE FORMING APPARATUS
First Claim
1. An image forming method for a biological sample, comprising:
- capturing optical images of a biological sample formed by a microscope that converts a phase distribution into an image intensity distribution while changing an image contrast, to form a plurality of pieces of images with different image contrasts;
calculating a component corresponding to a phase distribution of the biological sample and a component corresponding to a matter other than the phase distribution of the biological sample according to the plurality of pieces of images, and forming a normalized phase component image by dividing the component corresponding to the phase distribution by the component corresponding to the matter other than the phase distribution of the biological sample;
separating the phase component image into a plurality of frequency components according to spatial frequencies of the image;
applying a deconvolution process to each of the frequency components using an optical response characteristic corresponding to each, to calculate a phase distribution of a refraction component formed by light refracted inside the biological sample and a phase distribution of a structure component formed by light diffracted in a structure inside the biological sample;
merging the phase distribution of the refraction component and the phase distribution of the structure component to calculate the phase distribution of the biological sample, and forming a phase distribution image from the calculated phase distribution of the biological sample; and
merging the phase distribution image of the biological sample with an image of the biological sample in which a biochemical phenomenon and/or a physical phenomenon in the biological sample are visualized and which is obtained using a method that is different from a method used for the phase distribution image.
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Accused Products
Abstract
An image forming method for a biological sample includes calculating a component corresponding to a phase distribution of the sample and a component corresponding to a matter other than the phase distribution according to a plurality of pieces of images with different image contrasts, to form a normalized phase component; separating the phase component image into a plurality of frequency components according to spatial frequencies of the image; merging the phase distribution of the refraction component and the phase distribution of the structure component calculated by applying a deconvolution process to each of the frequency components using an optical response characteristic corresponding to each, to calculate the phase distribution, and forming a phase distribution image from the calculated phase distribution; and merging the phase distribution image with an image of the sample in which a biochemical phenomenon and/or a physical phenomenon in the sample are visualized.
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Citations
15 Claims
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1. An image forming method for a biological sample, comprising:
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capturing optical images of a biological sample formed by a microscope that converts a phase distribution into an image intensity distribution while changing an image contrast, to form a plurality of pieces of images with different image contrasts; calculating a component corresponding to a phase distribution of the biological sample and a component corresponding to a matter other than the phase distribution of the biological sample according to the plurality of pieces of images, and forming a normalized phase component image by dividing the component corresponding to the phase distribution by the component corresponding to the matter other than the phase distribution of the biological sample; separating the phase component image into a plurality of frequency components according to spatial frequencies of the image; applying a deconvolution process to each of the frequency components using an optical response characteristic corresponding to each, to calculate a phase distribution of a refraction component formed by light refracted inside the biological sample and a phase distribution of a structure component formed by light diffracted in a structure inside the biological sample; merging the phase distribution of the refraction component and the phase distribution of the structure component to calculate the phase distribution of the biological sample, and forming a phase distribution image from the calculated phase distribution of the biological sample; and merging the phase distribution image of the biological sample with an image of the biological sample in which a biochemical phenomenon and/or a physical phenomenon in the biological sample are visualized and which is obtained using a method that is different from a method used for the phase distribution image. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. An image forming apparatus comprising:
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a microscope that converts a phase distribution of a biological sample into an image intensity distribution and that includes an image contrast changing unit which changes an image contrast of the image intensity distribution; a control unit which controls the image contrast changing unit so as to obtain a plurality of pieces of images with different image contrasts; an operating unit which calculates a component corresponding to the phase distribution of the biological sample and a component corresponding to a matter other than the phase distribution of the biological sample according to the plurality of pieces of images obtained with control by the control unit, and forms a normalized phase component image by dividing the component corresponding to the phase distribution by the component corresponding to the matter other than the phase distribution of the biological sample;
separates the phase component image into a plurality of frequency components according to spatial frequencies of the image;
applies a deconvolution process to each of the frequency components using an optical response characteristic corresponding to each, to calculate a phase distribution of a refraction component formed by light refracted inside the biological sample and a phase distribution of a structure component formed by light diffracted in a structure inside the biological sample; and
merges the phase distribution of the refraction component and the phase distribution of the structure component to calculate the phase distribution of the biological sample, and forms a phase distribution image from the calculated phase distribution of the biological sample; anda merging unit which merges an image of the biological sample in which a biochemical phenomenon and/or a physical phenomenon in the biological sample are visualized and which is obtained using a method that is different from a method used for the phase distribution image with the phase distribution image of the biological sample formed by the operating unit. - View Dependent Claims (11, 12, 13, 14, 15)
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Specification