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IMAGE FORMING METHOD AND IMAGE FORMING APPARATUS

  • US 20150185460A1
  • Filed: 12/15/2014
  • Published: 07/02/2015
  • Est. Priority Date: 12/26/2013
  • Status: Abandoned Application
First Claim
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1. An image forming method for a biological sample, comprising:

  • capturing optical images of a biological sample formed by a microscope that converts a phase distribution into an image intensity distribution while changing an image contrast, to form a plurality of pieces of images with different image contrasts;

    calculating a component corresponding to a phase distribution of the biological sample and a component corresponding to a matter other than the phase distribution of the biological sample according to the plurality of pieces of images, and forming a normalized phase component image by dividing the component corresponding to the phase distribution by the component corresponding to the matter other than the phase distribution of the biological sample;

    separating the phase component image into a plurality of frequency components according to spatial frequencies of the image;

    applying a deconvolution process to each of the frequency components using an optical response characteristic corresponding to each, to calculate a phase distribution of a refraction component formed by light refracted inside the biological sample and a phase distribution of a structure component formed by light diffracted in a structure inside the biological sample;

    merging the phase distribution of the refraction component and the phase distribution of the structure component to calculate the phase distribution of the biological sample, and forming a phase distribution image from the calculated phase distribution of the biological sample; and

    merging the phase distribution image of the biological sample with an image of the biological sample in which a biochemical phenomenon and/or a physical phenomenon in the biological sample are visualized and which is obtained using a method that is different from a method used for the phase distribution image.

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