Centrifugable liquid vessel integrating a magnetic-bead separation-device

• US 20150196905A1
• Filed: 01/13/2014
• Published: 07/16/2015
• Est. Priority Date: 01/15/2013
• Status: Abandoned Application

• Current Assignee: Robert Pinard
• Original Assignee: Robert Pinard
• Inventors: Pinard, Robert
• Application Number:
  US14/153,820
• Publication Number:
  US 20150196905A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

A centrifugable liquid vessel integrating a magnet in its lid and used for the separation of magnetic beads in suspension in a liquid. The device allows the separation of the magnetic beads and their efficient transfer or wash in the same or in a new vessel without the use of a pipetting device. The segregation, wash and transfer of the beads without aspiration significantly reduce the risks of contamination and maximize the magnetic beads recovery.

Claims

1. A centrifugable liquid vessel integrating a magnet in its lid and used for the separation of magnetic beads in suspension in a liquid and comprising of essentially four detachable components, a centrifugable liquid vessel, a small powerful removable magnet, a hollow cylindrical connector cap and an opened lid adapter.

2. Said the centrifugable liquid vessel of claim 1 possesses threaded fitting on the interior wall of the hollow cylindrical portion located at the bottom of the said vessel allowing the magnet-adapter with or without segregated magnetic beads to be screwed and stored underneath.

3. Said the centrifugable liquid vessel of claim 1 wherein the top and bottom circumferences are similar and possess a beveled shape allowing the device to be placed in an upright or inverted position during the centrifugation process.

4. Said the centrifugable liquid vessel of claim 1 wherein the uniform magnetic field is generated by at least one removable or permanent magnet in the lid of the vessel.

5. Said the centrifugable liquid vessel of claim 1 wherein the removable magnet component is composed essentially of an adapter with threaded fitting and a strong magnet

6. Said the centrifugable liquid vessel of claim 1 wherein the hollow cylindrical connector cap component is closed at one extremity and includes dual threaded fittings allowing the attachment of an opened lid-adapter at one end and the removable magnet at the other end.

7. Said the centrifugable liquid vessel of claim 1 wherein the opened lid-adapter component includes dual threaded fittings, one fitting located inside its narrow cylindrical portion to receive the hollow cylindrical cap connector and the other one within its wider portion for its fitting atop the centrifugable liquid vessel.