MODULAR POINT-OF-CARE DEVICES, SYSTEMS, AND USES THEREOF
5 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.
64 Citations
108 Claims
-
1-78. -78. (canceled)
-
79. A method of detecting at least two analytes present in a sample, the method comprising:
-
positioning a cartridge in an instrument, wherein the cartridge comprises; at least one sample vessel containing the sample;
a plurality of reagent units;
a plurality of assay units;
at least one pipette tip, and at least one touch-off pad, all of the foregoing arranged in a two-dimensional pattern and not together in a single row;processing the sample to provide processed sample portions in selected assay units of the cartridge, wherein a) at least one of said selected assays units contains a first processed sample portion comprising one or more reagents and at least a first diluted portion of the sample at a first dilution, and b) at least another of said selected assays units contains a second processed sample portion comprising one or more reagents and at least a second diluted portion of the sample at a second dilution, wherein concentration of analytes in the first diluted portion is a fraction of the concentration of analytes in the sample and wherein the first dilution is different from the second dilution; decoupling at least one of said selected assay units from the cartridge and transporting said at least one of said selected assay units to a signal detector at a different location in the instrument; using the signal detector to detect at least one signal from at least one of said analytes; repeating the using the signal detector step with said at least one of said selected assay units or a different assay unit until said at least two analytes are detected; and containing in the cartridge all reagents, supplies, and wastes used in detection of said at least two analytes. - View Dependent Claims (80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107)
-
-
108. A system for detecting at least two analytes present in a sample, the system comprising:
-
a cartridge comprising; at least one sample vessel containing the sample;
a plurality of reagent units;
a plurality of assay units;
at least one pipette tip, and at least one touch-off pad, all of the foregoing arranged in a two-dimensional pattern and not together in a single row;an instrument comprising; an automated fluid handling device; a cartridge receiving location configured to position the cartridge effective that the automated fluid transfer device can engage at least one sample vessel, one of said reagent units, one of said assay units, or at least one pipette tip in the cartridge; and a signal detector, wherein the fluid transfer device is used to decouple at least one of selected assay units from the cartridge and transport said at least one of said selected assay units to the signal detector at a different location in the instrument; wherein the cartridge is configured to contain all reagents, supplies, and wastes used in detection of said at least two analytes.
-
Specification