FLUIDIC VALVE WITH SELECTIVLY SWITCHABLE STORAGE PATHS

• US 20150204828A1
• Filed: 06/26/2012
• Published: 07/23/2015
• Est. Priority Date: 06/26/2012
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Witt, Klaus, Choikhet, Konstantin
• Granted Patent: US 9,335,309 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B01D 15/1871 placed in series
• CPC Class Codes: G01N 2030/208 with more than one cavity
• CPC Class Codes: G01N 27/447 using electrophoresis
• CPC Class Codes: G01N 30/06 Preparation
• CPC Class Codes: G01N 30/20 using a sampling valve
• CPC Class Codes: G01N 30/461 with serial coupling of separation columns
• CPC Class Codes: G01N 30/6039 in series
• CPC Class Codes: G01N 30/80 Fraction collectors

Abstract

A sample separation apparatus (100, 200) for separating a fluidic sample (104), the sample separation apparatus (100, 200) comprising: a first separation unit (102) for separating the fluidic sample (104); a first fluid drive (106) configured for generating a fluid flow for conducting the fluidic sample (104) to be separated through the first separation unit (102); a second separation unit (110), arranged downstream of the first separation unit (102), for further separating the fluidic sample (104) after treatment by the first separation unit (102); a second fluid drive (112) configured for generating a fluid flow for conducting the fluidic sample (104) or at least parts thereof, after treatment by the first separation unit (102), through the second separation unit (110); a fluidic valve (114) having a first inlet (116) fluidically coupled to one of the first fluid drive (106) and the second fluid drive (112); the fluidic valve (114) having a second inlet (118) fluidically coupled to the other of the first fluid drive (106) and the second fluid drive (112); the fluidic valve (114) comprising at least two different sets (131, 133) of storage paths, wherein each set of storage paths comprises a first storage path (128); and wherein the first storage path (128) of a first set (131) of said at least two sets of storage paths has a first volume and the first storage path (132) of a second set (133) of said at least two sets of storage paths has a second volume different from the first volume; the fluidic valve (114) being configured for selectively switching one set of said least two sets of storage paths to the first inlet (116) and the second inlet (118).

Claims

1. A sample separation apparatus for separating a fluidic sample, the sample separation apparatus comprising:

a first separation unit for separating the fluidic sample;

a first fluid drive configured for generating a fluid flow for conducting the fluidic sample to be separated through the first separation unit;

a second separation unit, arranged downstream of the first separation unit, for further separating the fluidic sample after treatment by the first separation unit;

a second fluid drive configured for generating a fluid flow for conducting the fluidic sample or at least parts thereof, after treatment by the first separation unit, through the second separation unit;

a fluidic valve having a first inlet fluidically coupled to one of the first fluid drive and the second fluid drive;

the fluidic valve having a second inlet fluidically coupled to the other of the first fluid drive and the second fluid drive;

the fluidic valve comprising at least two different sets of storage paths, wherein each set of storage paths comprises a first storage path; and

wherein the first storage path of a first set of said at least two sets of storage paths has a first volume and the first storage path of a second set of said at least two sets of storage paths has a second volume different from the first volume;

the fluidic valve being configured for selectively switching one set of said at least two sets of storage paths to the first inlet and the second inlet.

2. The sample separation apparatus according to claim 1, the fluidic valve further comprising:

a first outlet and a second outlet;

the fluidic valve being selectively switchable into a first state and a second state, optionally comprising at least one of the following features;

in the first state the first inlet is fluidically coupled to the first outlet and the second inlet is fluidically coupled to the second outlet, wherein optionally the first inlet is fluidically coupled to the first outlet via the first storage path of the first set;

in the second state in the first inlet is fluidically coupled to the second outlet and the second inlet is fluidically coupled to the first outlet, wherein optionally the second inlet is fluidically coupled to the first outlet via the first storage path of the first set.

3. The sample separation apparatus according to claim 2, further comprising the following feature:

the fluidic valve being selectively switchable into the first state and into the second state for each of at least one set of said at least two sets of storage paths.

4. The sample separation apparatus according to claim 3, further comprising the following feature:

for each of at least one first set of said at least two sets of storage paths, the first outlet and the second outlet are both fluidically coupled to the second separation unit;

wherein optionally, first outlet and the second outlet are both permanently fluidically coupled to the second separation unit.

5. The sample separation apparatus according to claim 1, the fluidic valve further comprising:

a third outlet, wherein optionally the third outlet is a waste outlet bypassing the second separation unit;

the fluidic valve optionally further comprising at least one of the following groups of features;

for at least one of said sets, the fluidic valve is switchable into a third state in which the second inlet is fluidically coupled, via one of the first storage path and a second storage path of the set, to at least one of the first outlet the second outlet and in which the first inlet is fluidically coupled to the third outlet, optionally via the other of the first storage path and the second storage path of the set;

for at least one of said sets, the fluidic valve is switchable into a fourth state in which the first inlet is fluidically coupled, via one of the first storage path and the second storage path of the set, to at least one of the first outlet and the second outlet and the second inlet is fluidically coupled to the third outlet, optionally via the other of the first storage path and second storage path of the set.

6. The sample separation apparatus according to claim 2, wherein:

at least one set of said at least two different sets of storage paths comprises a second storage path;

wherein for each of the at least one set, if the set is switched to the first inlet and the second inlet, in the first state the first inlet is fluidically coupled to the first storage path of the set and the second inlet is fluidically coupled to the second storage path of the set; and

wherein for each of the at least one set, if the set is switched to the first inlet and the second inlet, in the second state the first inlet is fluidically coupled to the second storage path of the selected set and the second inlet is fluidically coupled to the first storage path of the selected set.

7. The sample separation apparatus according to claim 6, further comprising:

the fluidic valve being configured such that of the selected set of store paths, which is switched to the first inlet and to the second inlet, the first storage path is fluidically coupled to the first outlet and the second storage path is fluidically coupled to the second outlet in both, the first state and the second state.

8. The sample separation apparatus according to claim 2, comprising at least one of the following features:

a first base fluid path moveable to a first position corresponding to the first state and moveable to a second position corresponding to the second state of the fluidic valve;

the first base fluid path fluidically coupling the first inlet and the first outlet in the first state;

the first base fluid path fluidically coupling the first inlet and the second outlet in the second state;

a second base fluid path moveable to a first position corresponding to the first state and moveable to a second position corresponding to the second state of the fluidic valve;

the second base fluid path fluidically coupling the second inlet and the second outlet in the first state;

the second base fluid path fluidically coupling the second inlet and the first outlet in the second state.

9. The sample separation apparatus according to claim 8, wherein the first base fluid path comprises:

a first port region fluidically coupled to the first inlet;

a second port region moveable to be fluidically coupled to the first outlet in the first state and moveable to be fluidically closed in the second state; and

a third port region moveable to be fluidically coupled to the second outlet in the second state and moveable to be closed in the first state,

wherein the first port region, the second port region and the third port region of the first base fluid path are fluidically coupled to each other.

10. The sample separation apparatus according to claim 8, Wherein the second base fluid path comprises:

a first port region fluidically coupled to the second inlet;

a second port region moveable to be fluidically coupled to the second outlet in the first state and moveable to be fluidically closed in the second state; and

a third port region moveable to be fluidically coupled to the first outlet in the second state and moveable to be closed in the first state;

wherein the first port region, the second port region and the third port region of the second base fluid path are fluidically coupled to each other.

11. The sample separation apparatus according to claim 8, further comprising at least one of the following features:

the first base fluid path and/or the second base fluid path being a groove in a moveable member;

the first base fluid path and the second base fluid path are provided in a single moveable member.

12. The sample separation apparatus according to claim 11, wherein the first storage path of the second set of storage paths includes at least one of the following features:

the second volume is larger than the first volume;

the first storage path of the second set of storage paths includes at least part of the first storage path of the first set of storage paths.

13. The sample separation apparatus according to claim 12,

wherein, if the first storage paths of the at least two sets are ordered so as to have ascending volumes, a first storage path of a next set, which has a volume that is larger than the volume of the first storage path of each of the preceding sets, includes the first storage path of the preceding set and an additional storage path element;

and wherein optionally the first storage path of the first set, of which the first storage path has the smallest volume, includes only a single storage path element.

14. The sample separation apparatus according to claim 13, the fluidic valve further comprising:

a moveable coupling element;

the moveable coupling element being moveable into at least two coupling positions of which each coupling position corresponds to a selection of one of the at least two sets of storage paths.

15. The sample separation apparatus according to claim 13 and further comprising the features of claim 14, wherein each additional storage path element of the at least two sets has a first port and a second port, the fluidic valve further comprising:

the moveable coupling element having at least one coupling fluid path;

wherein in each coupling position the at least one coupling fluid path fluidically couples the storage paths elements of all preceding sets and the additional storage path element of the set corresponding to the coupling position via their first ports and their second ports, thereby providing the first storage path of the set of storage paths corresponding to the coupling position;

wherein optionally one of the first coupling fluid path and the second coupling fluid path fluidically couples the first storage path of the set of storage paths corresponding to the coupling-position to an outlet port; and

wherein optionally the at least one coupling fluid path is a groove in the moveable coupling element.

16. The sample separation apparatus according to claim 15, wherein the movement of the moveable coupling element defines a path of motion of the at least one coupling fluid path, the sample separation apparatus further comprising at least one of the following features:

the first port and the second port of each first storage path element is located in a position overlapping the path of motion of the at least one coupling fluid path;

the extent of the coupling fluid path in a direction along its path of motion is larger than the distance between the ports, to be coupled by the coupling fluid path, along the path of motion, wherein optionally of a first port and a second port to be coupled by the coupling fluid path at least a part of the first port is in line with the second port along the path of motion;

the extent of the coupling fluid path perpendicular to the path of motion is larger than the distance, perpendicular to the path of motion, between the ports to be coupled by the coupling fluid path, wherein optionally of a first port and a second port to be coupled by the coupling fluid path at least a part of the first port is in line with the second port perpendicular to the path of motion.

17. The sample separation apparatus according to claim 8, wherein

at least one of the first base fluid path and the second base fluid path is provided in the moveable coupling element.

18. The sample separation apparatus according to claim 17, further comprising one of the following features:

the extent of a coupling fluid path along the path of motion of the coupling fluid path is at least the distance along the path of motion between the ports to be coupled by the coupling fluid path plus the larger one of i) the distance along the path of motion between the first position and the second position of the first base fluid path and ii) the distance along the path of motion between the first position and the second position of the second base fluid path;

the at least one coupling fluid path comprising a first coupling fluid path and a second coupling fluid path different from the first coupling fluid path;

in the first position of the first base fluid path a first port and a second port are coupled by the first coupling fluid path, and in the second position of the first base fluid path the first port and the second port are coupler by the second coupling fluid path.

19. The sample separation apparatus according to claim 8, and further comprising at least one of the following features:

the first base fluid path is provided in a first switching element, the first switching element being moveable with regard to the moveable coupling element in order to switch one port of two end ports of the first storage path of the selected set into fluid communication with the first base fluid path or out of fluid communication with the first base fluid path;

the second base fluid path is provided in a second switching element, the second switching element being moveable with regard to the moveable coupling element in order to switch one port of two end ports of the first storage path of the selected set into fluid communication with the second base fluid path or out of fluid communication with the second base fluid path;

wherein optionally the second switching element is the first switching element;

wherein optionally the sample separation apparatus comprises an actuator for driving the first switching element; and

wherein optionally the first switching element comprises a stop face configured to be driveable, by the actuator, into contact with a corresponding stop face on the moveable coupling element, the moveable coupling element thereby being drivable by the first switching element upon further driving of the first switching element.

20. The sample separation apparatus according to claim 14, further comprising at least one of the following features:

the moveable coupling element is rotatable and is moveable into the at least two coupling positions by rotation of the moveable coupling element;

wherein optionally the sample separation apparatus comprises the features of claim 15 and the further features that the rotation of the moveable coupling element defines a circumferential direction and a radial direction perpendicular to the circumferential direction and that the at least one coupling fluid path comprises a circumferential groove section;

wherein optionally sample separation apparatus comprises the further features that the at least one coupling fluid path comprises a radial groove extending in a radial direction;

the moveable coupling element is linearly moveable coupling element being moveable into the at least two coupling positions along a linear axis.

21. The sample separation apparatus according to claim 20, further comprising at least one of the following features:

the sample separation apparatus comprises at least one actuator for controllably moving moveable elements;

the sample separation apparatus comprising a flow coupler having two fluid inlet terminals and a fluid outlet terminal in fluid communication with one another, the fluid outlet terminal being fluidically coupled or coupleable to the second separation unit;

wherein optionally the flow coupler is configured as one of the group consisting of a fluidic T-piece, a fluidic Y-piece, and a fluidic X-piece;

wherein the first separation unit is arranged between the first fluid drive and one of the first inlet and the second inlet of the fluidic valve which fluidically couples the first fluid drive with the fluidic valve;

wherein the second separation unit is directly fluidically coupled to the fluid outlet terminal of the flow coupler;

wherein the fluidic valve is switchable so that pressure conditions in the first separation unit and in the second separation unit remain constant before and after switching;

the sample separation apparatus comprises a detector for detecting the separated fluidic sample and being arranged downstream of the second separation unit;

the sample separation apparatus comprises a sample injector for injecting the fluidic sample into a mobile phase and being arranged between the first fluid drive and the first separation unit;

wherein the first fluid drive is operable with a first flow rate being smaller than a second flow rate according to which the second fluid drive is operable;

wherein optionally the second flow rate is at least five times, particularly is at least ten times, more particularly is at least fifty times, of the first flow rate;

wherein the fluidic valve is switchable for performing the separation of the fluidic sample so that the first fluid drive and the second fluid drive are in fluid communication with one another via the flow coupler in at least some of the switching states of the fluidic valve.

22. The sample separation apparatus according to claim 21, comprising a control device configured for:

controlling the first separation unit to execute a primary separation sequence within a measurement volume interval for separating the fluidic sample into a plurality of fractions;

controlling the second separation unit to execute a plurality of secondary separation sequences within the measurement volume interval for further separating at least a part of the plurality of separated fractions;

wherein optionally at least one of the primary separation sequence and the plurality of secondary separation sequences relates to a chromatographic gradient run.

23. The sample separation apparatus according to claim 22, further comprising at least one of the following:

the first separation unit and the second separation unit are configured so as to execute the respective sample separation in accordance with different separation criteria, particularly in accordance with at least partially but not completely orthogonal separation criteria, more particularly having different selectivity patterns to the relevant sample components;

the first separation unit and the second separation unit are configured so as to execute the respective sample separation on identical separation media but with different operating conditions, particularly at least one of the group consisting of different solvents, different steepness of elution gradients, different separation unit temperatures, and different pressures, so that the separation criteria are partially but not completely orthogonal.

24. The sample separation apparatus according to claim 23, comprising at least one of the following features:

the sample separation apparatus comprises a control device configured for controlling operation of at least one of the group consisting of the first fluid drive, the second fluid drive and the fluidic valve;

at least one of the first separation unit and the second separation unit is configured for performing a separation in accordance with one of the group consisting of liquid chromatography, supercritical-fluid chromatography, capillary electrochromatography, electrophoresis and gas chromatography;

the sample separation apparatus is configured as a two dimensional liquid chromatography sample separation apparatus, particularly being a comprehensive two-dimensional liquid chromatography apparatus;

the sample separation apparatus is configured to analyze at least one physical, chemical and/or biological parameter of at least one compound of the fluidic sample;

the sample separation apparatus comprises at least one of the group consisting of a chromatography device, a liquid chromatography device, an HPLC device, a gas chromatography device, a capillary electrochromatography device, an electrophoresis device, a capillary electrophoresis device, and a gel electrophoresis device;

the sample separation apparatus is configured for generating a fluid flow for conducting the fluidic sample with a high pressure;

the sample separation apparatus is configured for generating a fluid flow for conducting the fluidic sample with a pressure of at least 100 bar, particularly of at least 500 bar, more particularly of at least 1000 bar;

the sample separation apparatus is configured to conduct a liquid fluid;

the sample separation apparatus is configured as a microfluidic device;

the sample separation apparatus is configured as a nanofluidic device;

at least one of the group consisting of the first separation unit and the second separation unit is configured for retaining at least a part of components of the fluidic sample and for allowing other components of the fluidic sample to pass;

at least one of the group consisting of the first separation unit and the second separation unit comprises a separation column;

at least one of the group consisting of the first separation unit and the second separation unit comprises a chromatographic column;

at least a part of at least one of the group consisting of the first separation unit and the second separation unit is filled with a separating material.

25-27. -27. (canceled)