DEVICE AND METHOD FOR REAL-TIME DETECTION OF MOLECULAR ACCUMULATIONS AND/OR MONITORING THE PRODUCTION PROCESS OF A MOLECULAR MICROARRAY
First Claim
1. A method for producing a microarray, comprisinga) providing a first original array comprising a first support surface, to which at least one template molecule is bound,b) providing a second support surface (receiver surface), which is brought into a spatial orientation relative to the first support surfacec) providing a liquid, containing an enzymatic and/or chemical reaction system, between the first and second support surfaces, so that regions of the two surfaces are in contact with one another via the liquid,d) producing a product molecule using the template molecule and the enzymatic and/or chemical reaction system,e) transferring the product molecule to the second support surface via the liquid between the first and second support surfaces, wherein a correlation exists between a binding site of the template molecule to the first support surface and an accumulation of the corresponding product molecule on the second support surface, andf) detecting the accumulation of the product molecule on the receiver surface in real time.
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Accused Products
Abstract
The invention relates to a method for producing a microarray, wherein the production of this array is detected in real time from the accumulation of the product molecules being produced. The invention further relates to a microarray produced by this method, and to a device for the real-time detection of molecular accumulations on an array surface during the production of microarrays.
1 Citation
19 Claims
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1. A method for producing a microarray, comprising
a) providing a first original array comprising a first support surface, to which at least one template molecule is bound, b) providing a second support surface (receiver surface), which is brought into a spatial orientation relative to the first support surface c) providing a liquid, containing an enzymatic and/or chemical reaction system, between the first and second support surfaces, so that regions of the two surfaces are in contact with one another via the liquid, d) producing a product molecule using the template molecule and the enzymatic and/or chemical reaction system, e) transferring the product molecule to the second support surface via the liquid between the first and second support surfaces, wherein a correlation exists between a binding site of the template molecule to the first support surface and an accumulation of the corresponding product molecule on the second support surface, and f) detecting the accumulation of the product molecule on the receiver surface in real time.
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17. A device for the real-time detection of molecular accumulations on a receiver surface, comprising
a device for producing a microarray via of an enzymatic reaction and at least one detection device adapted to detect and/or measure an accumulation of a product molecule in real time.
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18. A kit for producing a microarray, wherein production is detected in real time, comprising
a first support surface, at least one template molecule, a receiver surface, an enzymatic and/or chemical reaction system, and m. at least one detection device, which can detect and/or measure a product molecule accumulations in real time.
Specification