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METHODS OF PREPARING NUCLEIC ACIDS FOR SEQUENCING

  • US 20150211050A1
  • Filed: 01/26/2015
  • Published: 07/30/2015
  • Est. Priority Date: 01/27/2014
  • Status: Active Grant
First Claim
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1. A method of preparing nucleic acids for analysis, the method comprising:

  • (a) contacting a nucleic acid template comprising a first strand of a target nucleic acid with a complementary target-specific primer that comprises a target-specific hybridization sequence, under conditions to promote template-specific hybridization and extension of the target-specific primer; and

    (b) contacting a nucleic acid template comprising a second strand that is complementary to the first strand of the target nucleic acid with a plurality of different primers that share a common sequence that is 5′

    to different hybridization sequences, under conditions to promote template-specific hybridization and extension of at least one of the plurality of different primers,wherein an extension product is generated to contain both a sequence that is characteristic of the target-specific primer and a sequence that is characteristic of the at least one of the plurality of different primers.

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