MANIPULATION OF MICROPARTICLES IN LOW FIELD DIELECTROPHORETIC REGIONS
First Claim
Patent Images
1. A method for isolating a target biological material from a sample, the method comprising:
- a. applying the sample to a device, the device comprising an array of electrodes;
b. creating dielectrophoretic (DEP) low-field and dielectrophoretic (DEP) high-field regions on the array; and
c. selectively retaining the target biological material on the DEP low-field region.
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Abstract
The present invention includes methods, devices and systems for isolating a target biological material from a biological sample. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in isolated target biological material from complex fluids such as blood or environmental samples.
20 Citations
56 Claims
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1. A method for isolating a target biological material from a sample, the method comprising:
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a. applying the sample to a device, the device comprising an array of electrodes; b. creating dielectrophoretic (DEP) low-field and dielectrophoretic (DEP) high-field regions on the array; and c. selectively retaining the target biological material on the DEP low-field region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 45)
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38. A method of testing a subject for the presence or absence of a biological material, the method comprising:
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a. obtaining a sample from the subject; b. optionally centrifuging the sample to separate intact cells from the sample; c. applying the sample to a device comprising an array of electrodes; d. creating DEP low-field and DEP high-field regions on the array; e. selectively retaining material on the DEP low-field region; f. optionally isolating the retained material; g. analyzing the retained material; and h. determining the presence or absence of the biological material. - View Dependent Claims (39, 40, 41)
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42. A method of testing an industrial sample for the presence or absence of a biological material, the method comprising:
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a. obtaining the industrial sample; b. applying the sample to a device comprising an array of electrodes; c. creating DEP low-field and DEP high-field regions on the array; d. selectively retaining material on the DEP low-field region; e. optionally isolating the retained material; f. analyzing the biological material; and g. determining the presence or absence of the biological material.
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43. A method of diagnosing a disease in a subject, the method comprising:
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a. obtaining a sample from the subject; b. optionally centrifuging the sample to separate cells from the sample; c. applying the sample to a device comprising an array of electrodes; d. creating DEP low-field and DEP high-field regions on the array; e. selectively retaining the biological material on the DEP low-field region; f. testing the biological material for the presence of one or more biomarkers; and g. detecting the presence of one or more biomarkers in the sample, wherein the detection of the biomarker is indicative of the disease. - View Dependent Claims (44)
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46. An alternating current electrokinetic device for isolating target biological material from a sample, the device comprising:
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a. a housing b. a plurality of alternating current (AC) electrodes within the housing, the AC electrodes configured o be selectively energized to establish dielectrophoretic (DEP) high-field and dielectrophoretic (DEP) low-field regions, whereby AC electrokinetic effects provide for separation of the target biological material from other entities in the sample at the DEP low-field region of the device. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53)
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- 54. Isolated biological material that is selectively retained on a DEP low-field region of an alternating current electrokinetic device.
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56. A system for isolating target biological material from a sample, the system comprising:
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a. a device comprising a plurality of alternating current (AC) electrodes within the housing, the AC electrodes configured o be selectively energized to establish dielectrophoretic (DEP) high-field and dielectrophoretic (DEP) low-field regions, whereby AC electrokinetic effects provide for separation of the target biological material from other entities in the sample at the DEP low-field region of the device; wherein the biological material is at least 800 nm in diameter.
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Specification