CAPSULE ARRAY DEVICES AND METHODS OF USE
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Abstract
This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.
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Citations
101 Claims
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1-77. -77. (canceled)
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78. A method for generating a droplet, comprising:
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(a) combining a microcapsule comprising an oligonucleotide barcode and a nucleic acid analyte each in an aqueous phase at a first junction of two or more channels of a microfluidic device to form an aqueous mixture comprising said microcapsule and said nucleic acid analyte, wherein said microcapsule is degradable upon application of a stimulus; and (b) generating a droplet comprising said microcapsule and said nucleic acid analyte by contacting said aqueous mixture with an immiscible continuous phase at a second junction of two or more channels of said microfluidic device. - View Dependent Claims (79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101)
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Specification