×

RECORDING AND MAPPING LINEAGE INFORMATION AND MOLECULAR EVENTS IN INDIVIDUAL CELLS

  • US 20150225801A1
  • Filed: 02/11/2015
  • Published: 08/13/2015
  • Est. Priority Date: 02/11/2014
  • Status: Abandoned Application
First Claim
Patent Images

1. A method for characterizing lineage information or recording molecular events among cells in a cell population, comprising:

  • introducing, over a time period of multiple cell cycle generations, a plurality of molecular changes in at least one of one or more genetic scratchpads in one or more cells in a cell population,wherein the cell population comprises cells that have developed for one or more cell cycle generations,wherein each genetic scratchpad in the one or more genetic scratchpads comprises a polynucleotide sequence and a plurality of target sites within the polynucleotide sequence, andwherein each of the plurality of molecular changes is associated with a target site among the plurality of target sites;

    characterizing, at one or more time points during the time period, a status of molecular changes at each time point for the plurality of target sites in each genetic scratchpad in cells in the cell population, wherein the cells are essentially intact or undisrupted, wherein at least one time point in the one or more time points is two or more cell cycle generations from the beginning of the time period; and

    establishing lineage connections or a sequence of molecular changes between cells from different cell cycle generations by comparing statuses of molecular changes of the cells, wherein the molecular changes may represent one or more molecular events.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×