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METHOD FOR DETERMINING THE CONCENTRATION OF AN ANALYTE IN A LIQUID SAMPLE USING SMALL VOLUME SAMPLES AND FAST TEST TIMES

  • US 20150226693A1
  • Filed: 04/24/2015
  • Published: 08/13/2015
  • Est. Priority Date: 11/16/2001
  • Status: Active Grant
First Claim
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1. A method of determining the concentration of glucose in a blood sample, comprising the acts of:

  • providing a disposable, electrochemical biosensor test strip including a sample receiving cavity having a volume from about 0.3 μ

    l to less than 1 μ

    l, and a reagent within the sample receiving cavity, the reagent including an enzyme and a mediator;

    applying a blood sample containing glucose into the sample receiving cavity and into contact with the reagent to cause the blood sample to at least partially solubilize or hydrate the reagent, the glucose reacting with the reagent to produce an electroactive reaction product;

    detecting the blood sample in the sample receiving cavity;

    following said detecting, applying or controlling the potential across the sample receiving cavity producing an assay current;

    within the time period from about 3.5 seconds to about 6 seconds after said detecting, measuring the assay current at one or more times and thereby obtaining one or more assay current measurements, andwithin the time period from about 3.5 seconds to about 6 seconds after said detecting, determining the concentration of glucose in the blood sample using the one or more assay current measurements.

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