METHOD AND DETECTION SYSTEM FOR DETECTING AN ANALYTE

• US 20150233836A1
• Filed: 05/06/2015
• Published: 08/20/2015
• Est. Priority Date: 12/27/2012
• Status: Active Grant

• Current Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: OKUMURA, Yasuaki, KAWAMURA, Tatsurou, SHIOI, Masahiko, MINAMIGUCHI, Masaru
• Granted Patent: US 9,964,491 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/14503 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors A61B5/1459, A61B5/1464, A61B5/1473, A61B5/1482, A61B5/14865 take precedence
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 2201/12 Circuits of general importance; Signal processing

Abstract

An exemplary sensor chip includes a substrate, a metal pattern formed on a side of the substrate that is irradiated with excitation light, and a first substance and a second substance provided near the metal pattern. A first intensity Xa of the first surface-enhanced Raman-scattered light from the first substance and a second intensity Xb of the second surface-enhanced Raman-scattered light from the second substance are detected. An intensity ratio Xc, as obtained by dividing the second intensity Xb with the first intensity Xa, is calculated.

Claims

1. A method for detecting an analyte, comprising:

providing a detection apparatus,

the detection apparatus including a light source and a spectrometer;

irradiating a sensor chip with excitation light from the light source to generate first surface-enhanced Raman-scattered light having a first peak and second surface-enhanced Raman-scattered light having a second peak different from the first peak,

the sensor chip including

a substrate,

a metal pattern formed on a side of the substrate that is irradiated with the excitation light, and

a first substance and a second substance provided near the metal pattern, wherein,

the first substance generates the first surface-enhanced Raman-scattered light;

a first intensity Xa which is an intensity of the first surface-enhanced Raman-scattered light does not vary with changes in the concentration of the analyte;

the second substance generates the second surface-enhanced Raman-scattered light; and

a second intensity Xb which is an intensity of the second surface-enhanced Raman-scattered light varies with changes in the concentration of the analyte;

detecting the first surface-enhanced Raman-scattered light from the first substance with the spectrometer to obtain the first intensity Xa;

simultaneously with detecting the first surface-enhanced Raman-scattered light, detecting the second surface-enhanced Raman-scattered light from the second substance with the spectrometer to obtain the second intensity Xb; and

calculating a value Xc resulting from dividing the second intensity Xb with the first intensity Xa having been measured simultaneously with the second intensity Xb.

2. The method of claim 1, wherein,

the detection apparatus includes a memory retaining information indicating correlation between the value Xc and the concentration of the analyte;

and

the step of calculating the value Xc comprises calculating the concentration of the analyte based on the value Xc and the information.

3. The method of claim 2, wherein,

the sensor chip is embedded in a biological body;

and

the concentration of the analyte contained in the biological body is calculated.

4. The method of claim 1, wherein the analyte is glucose.

5. The method of claim 4, wherein,

the first substance is monochloro-para-xylylene polymer;

and

the second substance is 4-mercaptophenylboronic acid.

6. The method of claim 5, wherein,

the first peak of the first surface-enhanced Raman-scattered light is at 1209 cm⁻

1; and

the second peak of the second surface-enhanced Raman-scattered light is at 1076 cm<sup>−

1</sup>.

7. A detection system for detecting an analyte, comprising:

a detection apparatus, and

a sensor chip,

the detection apparatus including a light source and a spectrometer, wherein

the sensor chip includes

a substrate,

a metal pattern formed on a side of the substrate that is irradiated with excitation light from the light source, and

a first substance and a second substance provided near the metal pattern;

the first substance generates first surface-enhanced Raman-scattered light having a first peak;

a first intensity Xa which is an intensity of the first surface-enhanced Raman-scattered light does not vary with changes in the concentration of the analyte;

the second substance generates second surface-enhanced Raman-scattered light having a second peak different from the first peak;

a second intensity Xb which is an intensity of the second surface-enhanced Raman-scattered light varies with changes in the concentration of the analyte; and

the detection system

irradiates the sensor chip with the excitation light to generate the first surface-enhanced Raman-scattered light and the second surface-enhanced Raman-scattered light,

detects the first surface-enhanced Raman-scattered light from the first substance with the spectrometer to obtain the first intensity Xa, and simultaneously detects the second surface-enhanced Raman-scattered light from the second substance with the spectrometer to obtain the second intensity Xb, and

calculates a value Xc resulting from dividing the second intensity Xb with the first intensity Xa having been measured simultaneously with the second intensity Xb.

8. The detection system of claim 7, wherein,

the detection apparatus includes a memory retaining information indicating correlation between the value Xc and the concentration of the analyte;

and

the detection system calculates the concentration of the analyte based on the value Xc and the information.

9. The detection system of claim 8, wherein,

the sensor chip is embedded in a biological body;

and

the detection system calculates the concentration of the analyte contained in the biological body.

10. The detection system of claim 7, wherein the analyte is glucose.

11. The detection system of claim 10, wherein,

the first substance is monochloro-para-xylylene polymer;

and

the second substance is 4-mercaptophenylboronic acid.

12. The detection system of claim 11, wherein,

the first peak of the first surface-enhanced Raman-scattered light is at 1209 cm⁻

1; and

the second peak of the second surface-enhanced Raman-scattered light is at 1076 cm<sup>−

1</sup>.