SEQUENCING METHOD USING MAGNIFYING TAGS
First Claim
Patent Images
1. A method of magnifying the signal associated with one or more bases in all or part of the sequence of a target nucleic acid molecule comprising at least the steps of:
- a) converting at least a portion of said target sequence to a form suitable for binding an adapter molecule, preferably to single stranded form;
b) binding to at least a portion of said region suitable for binding an adapter molecule, preferably said single stranded region, created in step a) an adapter molecule comprising one or more magnifying tags, or comprising a means for attaching one or more magnifying tags, which tags correspond to one or more bases of said target sequence, preferably corresponding to one or more bases of said region suitable for binding said adapter molecule, preferably said single stranded region, to which said adapter molecule binds or in proximity to said region;
c) optionally ligating said target molecule to said adapter molecule such that at least said magnifying tags remain associated with said target molecule;
d) repeating step a), wherein said region suitable for binding said adapter, preferably said single stranded region, which is created includes one or more bases not associated with a magnifying tag according to step b);
e) repeating steps b) to d) wherein said adapter molecule binds to an adjacent or overlapping region of said target molecule relative to the region to which the adapter molecule of the previous cycle bound wherein said magnifying tags of each cycle of steps a) to c) are ligated together.
6 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a method of sequencing all or part of a target nucleic acid molecule by determining the sequence of a portion of said nucleic acid molecule in addition to information regarding the position of said portion and in particular provides a new method of sequencing involving the magnification of one or more bases of said bases to aid identification.
-
Citations
25 Claims
-
1. A method of magnifying the signal associated with one or more bases in all or part of the sequence of a target nucleic acid molecule comprising at least the steps of:
-
a) converting at least a portion of said target sequence to a form suitable for binding an adapter molecule, preferably to single stranded form; b) binding to at least a portion of said region suitable for binding an adapter molecule, preferably said single stranded region, created in step a) an adapter molecule comprising one or more magnifying tags, or comprising a means for attaching one or more magnifying tags, which tags correspond to one or more bases of said target sequence, preferably corresponding to one or more bases of said region suitable for binding said adapter molecule, preferably said single stranded region, to which said adapter molecule binds or in proximity to said region; c) optionally ligating said target molecule to said adapter molecule such that at least said magnifying tags remain associated with said target molecule; d) repeating step a), wherein said region suitable for binding said adapter, preferably said single stranded region, which is created includes one or more bases not associated with a magnifying tag according to step b); e) repeating steps b) to d) wherein said adapter molecule binds to an adjacent or overlapping region of said target molecule relative to the region to which the adapter molecule of the previous cycle bound wherein said magnifying tags of each cycle of steps a) to c) are ligated together. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 22, 23, 24, 25)
-
-
16. A method of sequencing all or part of a target nucleic acid molecule comprising at least the steps of:
-
a) determining the sequence of a portion of said nucleic acid molecule; b) determining the position of said portion within said nucleic acid molecule; and c) combining the information obtained in steps a) and b) to obtain the sequence of said molecule. - View Dependent Claims (17, 18, 19, 21)
-
Specification