MICROSCOPE AND METHOD FOR CONTROLLING SAME

• US 20150253561A1
• Filed: 09/05/2013
• Published: 09/10/2015
• Est. Priority Date: 09/07/2012
• Status: Abandoned Application

• Current Assignee: NanoEn Tek Incorporated
• Original Assignee: NanoEn Tek Incorporated
• Inventors: Lee, Jae-Hwa, Ahn, Sang-Hwa
• Application Number:
  US14/425,333
• Publication Number:
  US 20150253561A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G02B 21/04 involving mirrors
• CPC Class Codes: G02B 21/08 Condensers
• CPC Class Codes: G02B 21/24 Base structure
• CPC Class Codes: G02B 21/361 Optical details, e.g. image relay to the camera or image sensor G02B21/364 takes precedence; illumination details G02B21/06 and subgroups
• CPC Class Codes: G02B 21/365 Control or image processing arrangements for digital or video microscopes G02B21/361, G02B21/362 take precedence

Abstract

Disclosed is a microscope and a method of controlling the same. According to the one or more of the embodiments of the present inventions relating to the microscope, an optical module for observing cells may be separated from a control module to be disposed in a narrow space such as the inside of an incubator or the inside of a clean bench, and is capable of observing cells while measuring a distribution of the cells (confluence) in a predetermined cycle.

Claims

1. A microscope comprising:

at least one optical module configured to form an image of a microscopic object placed on a stage, transform the image into an electric signal, and provide the electric signal; and

at least one control module configured to receive the electric signal from the optical module, output an image, and control the at least one optical module according to a command input from a user,

wherein the at least one optical module is separable from the control module to be independently disposed in a limited space.

2. The microscope of claim 1, wherein a plurality of optical modules are used to simultaneously observe growth of a plurality of cell aggregates, and

at least one control module is used to control the plurality of optical modules.

3. The microscope of claim 1, wherein the at least one optical module comprises:

a light source unit configured to emit light onto the microscopic object;

an object lens configured to form an image of the microscopic object placed on the stage; and

an image sensor on which light passing through the object lens is incident and which is configured to transform the light into an electric signal.

4. The microscope of claim 3, wherein the at least one optical module further comprises:

a first mirror configured to reflect, at a predetermined angle, the light emitted from the light source unit toward the microscopic object; and

a second mirror configured to reflect, at a predetermined angle, the light passing through the object lens toward the image sensor.

5. The microscope of claim 4, wherein the at least one optical module further comprises:

a diffuser configured to diffuse the light emitted from the light source unit;

a condensing lens configured to concentrate the light passing through the diffuser; and

a filter configured to filter the light passing through the condensing lens.

6. The microscope of claim 1, wherein the at least one optical module comprises at least one cooling device configured to circulate heat, which is generated in the at least one optical module, in the limited space so as to achieve thermal equilibrium.

7. The microscope of claim 1, wherein the at least one optical module and the at least one control module communicate with each other in a wireless or wired manner.

8. The microscope of claim 1, wherein the at least one control module comprises:

a display unit configured to receive the electric signal from the at least one optical module and output an image; and

a control unit configured to control the at least one optical module according to a command input from a user.

9. The microscope of claim 8, wherein the at least one control unit captures images of the microscopic object and stores the images in a memory device in a predetermined cycle which is set by a user.

10. The microscope of claim 9, wherein the control unit calculates a confluence which is a distribution of the cells based on the images of the microscopic object captured in the predetermined cycle and displays the confluence in the form of a graph or in the form of numerical values.

11. The microscope of claim 10, wherein the control unit transmits an alarm to a user when the confluence reaches a predetermined level.

12. The microscope of claim 9, wherein the control unit provides a video file by combining a file of the images of the microscopic object captured in the predetermined cycle according to a request from a user.

13. The microscope of claim 8, wherein the at least one optical module comprises a focus control unit configured to adjust a focal point of an image to be captured, and

the control unit controls the focus control unit to automatically control a focal point of an image as the cells grow.

14. A method of controlling a microscope in which an optical module for observing a microscopic object and a control module for controlling the optical module are separated from each other to independently dispose the optical module in a limited space, the method comprising:

capturing and storing images of a microscopic object in a predetermined cycle which is set by a user; and

analyzing the stored images to automatically measure a confluence which is a distribution of cells.

15. The method of claim 14, further comprising displaying a result of measuring the confluence in the form of a time-based graph or in the form of numerical values.

16. The method of claim 14, further comprising providing a video file by combining a file of the images of the microscopic object captured in the predetermined cycle according to a request from a user.

17. The method of claim 14, further comprising controlling a focus control unit to automatically control a focal point of an image as the cells which are the microscopic object grow.

18. The method of claim 14, further comprising transmitting an alarm to a user when the confluence reaches a predetermined level.