RAPID DETECTION AND QUANTITATION OF PATHOGEN-SPECIFIC BIOMARKERS USING NANOPOROUS DUAL- OR MULTI-LAYER SILICA FILMS
First Claim
1. A method of identifying at least one pathogen-specific peptide or polypeptide from a biological sample, comprising contacting the sample with a nanoporous dual- or multi-layer silica film;
- and detecting the presence of the pathogen-specific peptide or polypeptide, or one or more proteolytic fragment(s) thereof.
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Abstract
Improved methods for detecting active tuberculosis are disclosed. A method comprises enriching at least one M. tuberculosis-specific biomolecule from a sample by contacting the sample with a nanoporous film; and detecting the presence of the M. tuberculosis-specific biomolecule or fragment(s) thereof. The method may further comprise digesting the enriched M. tuberculosis-specific biomolecule with an enzyme to produce a digestion product comprising at least one fragment of the M. tuberculosis-specific biomolecule. Improved sensitivity and speed achieved.
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Citations
28 Claims
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1. A method of identifying at least one pathogen-specific peptide or polypeptide from a biological sample, comprising contacting the sample with a nanoporous dual- or multi-layer silica film;
- and detecting the presence of the pathogen-specific peptide or polypeptide, or one or more proteolytic fragment(s) thereof.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method, comprising:
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a) enriching at least one target protein or polypeptide from a sample by contacting the sample with a nanoporous dual- or multi-layer silica film under conditions to absorb the target protein or polypeptide to the film, and subsequently washing the nanoporous dual- or multi-layer silica film to remove extraneous material; b) digesting the enriched target protein or polypeptide on the nanoporous dual- or multi-layer silica film to produce at least one digestion product comprising at least one proteolytic fragment thereof; and c) detecting the presence of the at least one proteolytic fragment of the target protein or polypeptide. - View Dependent Claims (24, 25, 26, 27, 28)
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Specification