Methods of Storing Information Using Nucleic Acids

• US 20150269313A1
• Filed: 07/17/2013
• Published: 09/24/2015
• Est. Priority Date: 07/19/2012
• Status: Active Grant

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Church, George M.
• Granted Patent: US 9,384,320 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: G06K 19/00 Record carriers for use with machines and with at least a part designed to carry digital markings
• CPC Class Codes: G06K 19/022 Processes or apparatus therefor
• CPC Class Codes: G06N 3/123 DNA computing
• CPC Class Codes: G16B 30/00 ICT specially adapted for sequence analysis involving nucleotides or amino acids
• CPC Class Codes: G16B 30/20 Sequence assembly

Abstract

The present invention relates to methods of storing data using one or more nucleic acids.

Claims

1. A method of storing information using nucleotides comprising

converting a format of information into a plurality of bit sequences of a bit stream with each having a corresponding bit barcode,

converting the plurality of bit sequences to a plurality of corresponding oligonucleotide sequences using one bit per base encoding,

synthesizing the plurality of corresponding oligonucleotide sequences, and

storing the synthesized plurality of corresponding oligonucleotide sequences.

2. The method of claim 1 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

3. A method of retrieving a format of information from a plurality of synthesized oligonucleotide sequences encoding bit sequences of the format of information comprising

amplifying the plurality of oligonucleotide sequences,

sequencing the amplified oligonucleotide sequences,

converting the oligonucleotide sequences to bit sequences, and

converting the bit sequences to the format of information.

4. The method of claim 3 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

5. A method of accessing a format of information from a plurality of synthesized oligonucleotide sequences encoding bit sequences of the format of information comprising

amplifying the plurality of oligonucleotide sequences,

sequencing the amplified oligonucleotide sequences,

converting the oligonucleotide sequences to bit sequences,

converting the bit sequences to the format of information, and

visualizing the format of information.

6. The method of claim 5 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

7. A method of storing information using nucleotides comprising converting a format of information to a bit stream, encoding first bit sequences into corresponding oligonucleotide sequences, synthesizing the oligonucleotide sequences, sequencing the oligonucleotide sequences, decoding the oligonucleotide sequences into bit sequences, assembling the bit sequences into a bit stream and converting the bit stream into the format of information.

8. The method of claim 7 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

9. A method of storing information using nucleotides comprising converting a format of information to a first bit stream, encoding first bit sequences into corresponding oligonucleotide sequences, synthesizing the oligonucleotide sequences, sequencing the oligonucleotide sequences, decoding the oligonucleotide sequences into second bit sequences, assembling the second bit sequences into a second bit stream and converting the second bit stream into the format of information.

10. The method of claim 9 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

11. A method of storing information using nucleotides comprising converting a first format of information to a first bit stream, encoding first bit sequences into corresponding oligonucleotide sequences, synthesizing the oligonucleotide sequences, sequencing the oligonucleotide sequences, decoding the oligonucleotide sequences into second bit sequences, assembling the second bit sequences into a second bit stream and converting the second bit stream into a second format of information.

12. The method of claim 11 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

13. A method of retrieving a format of information from a plurality of synthesized oligonucleotide sequences encoding bit sequences of the format of information comprising

sequencing the amplified oligonucleotide sequences,

converting the oligonucleotide sequences to bit sequences, and

converting the bit sequences to the format of information.

14. The method of claim 13 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

15. A method of accessing a format of information from a plurality of synthesized oligonucleotide sequences encoding bit sequences of the format of information comprising

sequencing the amplified oligonucleotide sequences,

converting the oligonucleotide sequences to bit sequences,

converting the bit sequences to the format of information, and

visualizing the format of information.

16. The method of claim 15 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.