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INDUCIBLE DNA BINDING PROTEINS AND GENOME PERTURBATION TOOLS AND APPLICATIONS THEREOF

  • US 20150291966A1
  • Filed: 01/23/2015
  • Published: 10/15/2015
  • Est. Priority Date: 07/25/2012
  • Status: Abandoned Application
First Claim
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1. An engineered, non-naturally occurring Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) (CRISPR-Cas) vector system comprising one or more vectors comprising:

  • a) a first regulatory element operably linked to one or more nucleotide sequences encoding one or more CRISPR-Cas system polynucleotide sequences comprising a guide sequence, a tracr RNA, and a tracr mate sequence, wherein the guide sequence hybridizes with one or more target sequences in polynucleotide loci in a eukaryotic cell,b) a second regulatory element operably linked to a nucleotide sequence encoding a Type II Cas9 protein,wherein components (a) and (b) are located on same or different vectors of the system,wherein the CRISPR-Cas system comprises at least one switch,whereby the activity of the system to target the one or more polynucleotide loci is controlled.

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