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SHORT CYCLE METHODS FOR SEQUENCING POLYNUCLEOTIDES

  • US 20150292008A1
  • Filed: 03/19/2015
  • Published: 10/15/2015
  • Est. Priority Date: 11/12/2003
  • Status: Active Grant
First Claim
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1. A method for obtaining sequence information from a plurality of different target nucleic acids immobilized at discrete locations on a surface, wherein a plurality of the target nucleic acids comprise a common adaptor sequence and have a primer hybridized thereto;

  • wherein the method comprises multiple cycles of the following steps;

    (a) contacting the target nucleic acids with a solution that contains an enzyme and a detectably labeled nucleotide such that the enzyme catalyzes template-dependent addition of the labeled nucleotide to extend a plurality of the primers,wherein the labeled nucleotide is not a chain-terminating nucleotide, andwherein addition of the labeled nucleotide to a primer interferes with progression of the enzyme;

    then(b) detecting addition of the labeled nucleotide to at least some of the primers, thereby indicating the presence of a complementary nucleotide in the target nucleic acid hybridized thereto.

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