MATURATION OF HEPATOCYTE-LIKE CELLS DERIVED FROM HUMAN PLURIPOTENT STEM CELLS
First Claim
1. A method for promoting the maturation of human hepatocyte-like cells, the method comprising:
- Exposing said human hepatocyte-like cells to an activator of a retinoic acid responsive receptor.
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Accused Products
Abstract
The present invention relates to directed differentiation and maturation of hepatocyte-like cells. In particular, the present invention relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of GSK-3 (Glycogen synthase kinase 3) or activator of Wnt signalling and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay). The present invention also relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of a cycline dependent kinase (CDK) and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay). The hepatocyte-like cells obtained in accordance with the present invention show a phenotype which is more similar to that of primary hepatocytes than previously shown.
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Citations
87 Claims
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1. A method for promoting the maturation of human hepatocyte-like cells, the method comprising:
Exposing said human hepatocyte-like cells to an activator of a retinoic acid responsive receptor. - View Dependent Claims (2, 5, 6, 7, 8, 9, 10, 11, 12, 13, 24, 34, 36, 43, 72)
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3-4. -4. (canceled)
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14-23. -23. (canceled)
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25-33. -33. (canceled)
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35. (canceled)
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37-42. -42. (canceled)
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44-71. -71. (canceled)
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73-78. -78. (canceled)
- 79. A kit comprising at least one activator of a retinoic acid responsive receptor and at least one item selected from the group consisting of GSK3 inhibitors, activators of Wnt signalling, CDK inhibitors and extracellular matrix (ECM) components or ECM component mixtures.
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81-84. -84. (canceled)
- 85. A composition comprising at least one activator of a retinoic acid responsive receptor and at least one item selected from the group consisting of GSK3 inhibitors, activators of Wnt signalling and CDK inhibitors.
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87-88. -88. (canceled)
Specification