MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL
First Claim
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1. A method of carrying out amplification on a plurality of samples, the method comprising:
- introducing each of the plurality of samples into an amplification zone of a multi-lane microfluidic cartridge;
isolating each of the plurality of samples within the multi-lane microfluidic cartridge;
independently thermally cycling each amplification zone; and
amplifying polynucleotides contained within each of the plurality of samples.
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Abstract
The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
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Citations
20 Claims
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1. A method of carrying out amplification on a plurality of samples, the method comprising:
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introducing each of the plurality of samples into an amplification zone of a multi-lane microfluidic cartridge; isolating each of the plurality of samples within the multi-lane microfluidic cartridge; independently thermally cycling each amplification zone; and amplifying polynucleotides contained within each of the plurality of samples. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of carrying out amplification on a plurality of samples, the method comprising:
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introducing a sample of a plurality of samples into an amplification zone in a lane of a multi-lane microfluidic cartridge; isolating the sample from the plurality of samples within the multi-lane microfluidic cartridge; thermally controlling the amplification zone independently from another amplification zone in another lane of the multi-lane microfluidic cartridge; and amplifying polynucleotides contained within the sample within the amplification zone while thermal cycling the amplification zone. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14)
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15. A method of carrying out nucleic acid amplification on a plurality of samples, the method comprising:
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introducing the plurality of samples into a plurality of amplification zones of a multi-lane microfluidic cartridge; isolating the plurality of samples within the plurality of amplification zones; and independently heating each amplification zone in order to amplify polynucleotides contained within the sample within the amplification zone. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification