AN APPARATUS FOR DETECTING A COMPONENT IN A SAMPLE

• US 20150316471A1
• Filed: 12/17/2013
• Published: 11/05/2015
• Est. Priority Date: 12/20/2012
• Status: Active Grant

• Current Assignee: Radiometer Medical ApS (Danaher Corporation)
• Original Assignee: Radiometer Medical ApS (Danaher Corporation)
• Inventors: ANDERSEN, Willy Lindegaard, HANSEN, Heine, DANNEVANG, Oluf, HANSEN, Ole Munch
• Granted Patent: US 9,683,931 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

An apparatus for detecting a first component in a sample, the first component being responsive to at least radiation of a first wavelength, the sample comprising the first component and a second component responsive to at least radiation of a second wavelength.

Claims

1. An apparatus for detecting a component in a sample comprising, a first component being responsive to at least radiation of a first wavelength, the sample comprising the first component and a second component responsive to at least radiation of a second wavelength, the apparatus comprising;

at least one radiation source configured to direct radiation towards the sample;

at least one radiation detector configured to detect radiation of at least the first and the second wavelength, said detected radiation having propagated along a radiation path through at least a portion of the sample; and

a processing unit operable to receive at least one detector signal from the at least one radiation detector indicative of the detected radiation, and to

determine an estimated path length of the radiation path at least from a determined absorbance by the sample of radiation at the second wavelength;

determine an estimated concentration of the first component at least from a determined absorbance by the sample of radiation at the first wavelength and from the estimated path length;

determine a corrected concentration of the first component at least from the estimated concentration and from a correction term indicative of a corrected path length corrected for a presence of the first component using the estimated concentration.

2. The apparatus according to claim 1 further comprising a sample chamber for accommodating the sample, the sample chamber defining the radiation path.

3. The apparatus according to claim 2, comprising an actuator operable to change the path length of the radiation path between at least a first and a second path length;

and wherein the processing unit is operable to determine a concentration of the first component from a difference of absorbance measurements at the first wavelength measured with the path length set to the first path length and absorbance measurements at the first wavelength measured with the path length set to the second path length.

4. The apparatus according to claim 3, wherein the processing unit is operable to

determine an estimated path length difference between the first and second path lengths at least from respective absorbance measurements at the second wavelength;

determine the estimated concentration of the first component at least from a difference of absorbance measurements at the first wavelength measured with the path length set to the first path length and absorbance measurements at the first wavelength measured with the path length set to the second path length, and from the estimated path length difference;

determine a corrected concentration of the first component at least from the estimated concentration and from a correction term indicative of a corrected path length difference between the first and second path lengths corrected for a presence of the first component using the estimated concentration.

5. The apparatus according to claim 1, wherein the processing unit is further operable to determine a concentration of a third component of the sample, the third component being responsive to at least radiation of the third wavelength, at least from the absorbance of the sample at the third wavelength, from the estimated path length, and from a correction term indicative of a corrected path length corrected for a presence of the first component using the estimated concentration of the first component.

6. The apparatus according to claim 1, wherein the processing unit is further operable to determine a concentration of a third component of the sample, the third component being responsive to at least radiation of a third wavelength, at least from the absorbance of the sample at the third wavelength and from the estimated path length.

7. The apparatus according to claim 1 wherein the at least one radiation source comprises a first radiation source configured to produce at least radiation at the first wavelength, and a second radiation source configured to produce at least radiation at the second wavelength.

8. The apparatus according to claim 7, comprising a beam combiner configured to direct radiation from the first radiation source and radiation from the second radiation source along a common radiation path through the sample.

9. The apparatus according to claim 1 wherein the at least one radiation detector comprises a first radiation detector configured to detect at least radiation at the first wavelength, and a second radiation detector configured to detect at least radiation at the second wavelength.

10. The apparatus according to claim 9, comprising a beam splitter configured to direct a first part of the radiation from the sample to the first radiation detector and a second part of the radiation from the sample to the second detector.

11. A method for determining a concentration of a component in a sample comprising, a first component being responsive to at least radiation of a first wavelength, the sample comprising the first component and a second component responsive to at least radiation of a second wavelength, the method comprising:

receiving a measured first absorbance of the sample at at least the first wavelength and a measured second absorbance of the sample at at least the second wavelength;

determining, at least from the second absorbance, an estimated path length of a radiation path along which the radiation has propagated through the sample;

determining an estimated concentration of the first component at least from the first absorbance and from the estimated path length;

determining a corrected concentration of the first component at least from the estimated concentration and from a correction term indicative of a corrected path length corrected for a presence of the first component using the estimated concentration.

12. The method according to claim 11, comprising determining the correction term from an absorption model for determining the second absorbance as being related to the path length by a suppression term, wherein the suppression term changes with changing concentration of the first component.

13. The method according to claim 12, comprising determining the correction term from an absorption model for determining the second absorbance as being proportional to the path length and related to the path length by a factor of proportionality, wherein the factor of proportionality decreases, between a maximum factor and a minimum factor, with increasing concentration of the first component.

14. The method according to claim 11, further comprising determining a concentration of a third component of the sample, the third component being responsive to at least radiation of a third wavelength, at least from the absorbance of the sample at the third wavelength, from the estimated path length, and from a correction term indicative of a corrected path length corrected for a presence of the first component using the estimated concentration of the first component.

15. The method according to claim 11, further comprising determining a concentration of a third component of the sample, the third component being responsive to at least radiation of a third wavelength, at least from the absorbance of the sample at the third wavelength and from the estimated path length.

27. The method according to claim 11, wherein the first component is an analyte and the second component is a solvent.

28. The method according to claim 27, wherein the solvent is water.

29. The method according to claim 27, wherein the analyte is chosen from total hemoglobin, bilirubin, and a derivative of hemoglobin.

30. The method according to claim 11, wherein determining the estimated path length comprises determining the estimated path length from the first absorbance and a predetermined proportionality factor.

31. A signal or data processing apparatus configured to perform the steps of the method of claim 11.

32. The signal or data processing apparatus according to claim 31, further comprising at least one radiation source configured to direct radiation towards a sample;

at least one radiation detector configured to detect radiation of at least the first and the second wavelength, said detected radiation having propagated along a radiation path through at least a portion of the sample.

33. A computer program comprising program code configured to cause a data processing system to perform the steps of the method of claim 11, when the program code is executed by the data processing system.

34. A computer readable medium having stored thereon a computer program according to claim 33.

16. A method for determining a path length of a radiation path along which radiation has propagated through a sample comprising:

the sample comprising a first component responsive to radiation at at least a first wavelength, and a second component responsive to radiation at at least a second wavelength, the method comprising;

receiving a measured first absorbance of the sample at at least the first wavelength, and a measured second absorbance of the sample at at least the second wavelength;

determining, at least from the second absorbance, an estimated path length of a radiation path along which the radiation has propagated through the sample;

determining an estimated concentration of the first component at least from the first absorbance and from the estimated path length;

correcting the estimated path length for a presence of the first component using the estimated concentration of the first component.

17. The method according to claim 16, wherein the estimated path length is corrected by a correction term determined from an absorption model for determining the second absorbance as being related to the path length by a model parameter, wherein the model parameter changes with changing concentration of the first component.

18. The method according to claim 17, wherein the correction term is determined from an absorption model for determining the second absorbance as being proportional to the path length where the factor of proportionality decreases, between a maximum factor and a minimum factor, with increasing concentration of the first component.

19. The method according to claim 16, wherein the first component is an analyte and the second component is a solvent.

20. The method according to claim 19, wherein the solvent is water.

21. The method according to claim 19, wherein the analyte is chosen from total hemoglobin, bilirubin, and a derivative of hemoglobin.

22. The method according to claim 16, wherein determining the estimated path length comprises determining the estimated path length from the first absorbance and a predetermined proportionality factor.

23. A signal or data processing apparatus configured to perform the steps of the method of claim 16.

24. The signal or data processing apparatus according to claim 23, further comprising at least one radiation source configured to direct radiation towards a sample;

at least one radiation detector configured to detect radiation of at least the first and the second wavelength, said detected radiation having propagated along a radiation path through at least a portion of the sample.

25. A computer program comprising program code configured to cause a data processing system to perform the steps of the method of claim 16, when the program code is executed by the data processing system.

26. A computer readable medium having stored thereon a computer program according to claim 25.