DEVICE AND METHODS FOR DETECTION OF ANALYTES INCLUDING USE OF A COLORIMETRIC BARCODE
First Claim
1. A device for determining an analyte, comprising:
- a sample collection portion arranged on a first region of the device and comprising a sample collection well;
a sample collection substrate detachably attached to the device and capable of being inserted into the sample collection well; and
a sample analysis portion arranged on a second region of the device and in fluid communication with the sample collection portion, the sample analysis portion comprising;
at least one fluid container containing a fluid carrier, at least one channel comprising a chemical reagent capable of generating a luminescent or colorimetric signal, and a mixing chamber in fluid communication with the at least one fluid container, the at least one channel, and the sample collection substrate,wherein the sample collection portion and the sample analysis portion are integrally connected to one another.
3 Assignments
0 Petitions
Accused Products
Abstract
Embodiments described herein related to devices and methods for the collection and/or determination of analytes, such as illicit substances including military explosives, explosives, and precursors thereof. In some cases, the device may be a disposable device that incorporates highly efficient sample collection in combination with microfluidic-based chemical analysis resulting in the rapid detection and identification of unknown materials. In some cases, multiple colorimetric detection chemistries may be employed, and the resulting “barcode” of color changes can be used to positively identify the presence and/or identity of the analyte.
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Citations
227 Claims
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1. A device for determining an analyte, comprising:
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a sample collection portion arranged on a first region of the device and comprising a sample collection well; a sample collection substrate detachably attached to the device and capable of being inserted into the sample collection well; and a sample analysis portion arranged on a second region of the device and in fluid communication with the sample collection portion, the sample analysis portion comprising;
at least one fluid container containing a fluid carrier, at least one channel comprising a chemical reagent capable of generating a luminescent or colorimetric signal, and a mixing chamber in fluid communication with the at least one fluid container, the at least one channel, and the sample collection substrate,wherein the sample collection portion and the sample analysis portion are integrally connected to one another. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 198, 199, 200, 201, 202, 203, 204, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227)
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35. A method for determining an analyte, comprising:
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providing a sample analysis device comprising at least 5 channels constructed and arranged to receive a sample suspected of containing an analyte, each channel comprising a different chemical reagent; and simultaneously introducing the sample into the at least 5 channels such that the analyte, if present, interacts with one or more of the chemical reagents to produce a determinable signal. - View Dependent Claims (36)
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71. A method for determining an analyte, comprising:
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providing a sample analysis device comprising a plurality of channels constructed and arranged to receive a sample suspected of containing an analyte, each channel comprising a different chemical reagent, each chemical reagent capable of generating a luminescent or colorimetric signal; and simultaneously introducing the sample into the plurality of channels such that the analyte, if present, interacts with one or more of the chemical reagents to produce a plurality of luminescent or colorimetric signals, thereby determining the analyte, wherein the presence and/or identity of the analyte is determined without need for a non-luminescent or non-colorimetric analysis of the sample. - View Dependent Claims (72)
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106. A method for determining an analyte, comprising:
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providing a sample collection device comprising a rolling substrate, the rolling substrate having a surface; contacting the surface of the rolling substrate with an article suspected of containing an analyte via a rolling or tapping motion such that a sample suspected of containing the analyte is collected on the surface of the rolling substrate; contacting the surface of the rolling substrate with a fluid carrier to transfer at least a portion of the sample to the fluid carrier to produce a fluid sample suspected of containing the analyte; introducing the fluid sample suspected of containing the analyte into a sample analysis device comprising a plurality of channels constructed and arranged to receive the fluid sample, wherein portions of the fluid sample are simultaneously introduced into each of a set of the plurality of channels, each of the set of channels comprising a different chemical reagent capable of generating a luminescent and/or colorimetric signal upon interaction with a specific analyte, wherein the analyte, if present in the fluid sample, interacts with one or more of the chemical reagents to produce a plurality of luminescent and/or colorimetric signals in at least some of the set of channels; and determining the presence and/or identity of the analyte based on the plurality of luminescent and/or colorimetric signals.
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140. A sample analysis device for determining an analyte, comprising:
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a sample inlet constructed and arranged to receive a sample suspected of containing an analyte; and at least 5 channels in fluid communication with the sample inlet and constructed and arranged for essentially simultaneous analysis of an analyte suspected of being present in a sample introduced into the inlet, each channel comprising a different chemical reagent in substantially solid form, wherein the analyte, if present, interacts with one or more of the chemical reagents to produce a determinable signal. - View Dependent Claims (141)
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168. A sample analysis device for determining an analyte, comprising:
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a sample inlet constructed and arranged to receive a sample suspected of containing an analyte; and a plurality of channels in fluid communication with the sample inlet, each channel comprising a different chemical reagent, each chemical reagent capable of generating a luminescent or colorimetric signal, wherein the analyte, if present, interacts with one or more of the chemical reagents to produce a plurality of luminescent or colorimetric signals, wherein the device does not include a crystallizing reagent. - View Dependent Claims (169)
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196. A kit for determining an analyte, comprising:
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a sample collection device, comprising; a first region comprising a fluid container containing a fluid carrier;
a second region comprising a surface constructed and arranged to contact an article suspected of containing the analyte and to receive fluid from the fluid container;
a sample outlet in fluid communication with the first and second regions; and
a housing containing the first region, the second region, and the sample outlet; anda sample analysis device, comprising; at least one sample inlet constructed and arranged to receive a sample suspected of containing an analyte from the sample outlet of the sample collection luminescent device; and
a channel in fluid communication with the sample inlet, the channel comprising a chemical reagent capable of generating a luminescent or colorimetric signal.- View Dependent Claims (197, 205, 206, 207, 208)
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Specification