Bilirubin Hematofluorometer and Reagent Kit

• US 20150323460A1
• Filed: 07/22/2015
• Published: 11/12/2015
• Est. Priority Date: 01/15/2013
• Status: Abandoned Application

• Current Assignee: Aviv Biomedical, Inc.
• Original Assignee: Aviv Biomedical, Inc.
• Inventors: Aviv, Jack, Lamola, Angelo A., Ramsay, Glen, MacDonald, Jeff
• Application Number:
  US14/806,178
• Publication Number:
  US 20150323460A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 2201/061 Sources
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 33/728 Bilirubin; including biliverdin

Abstract

A hematofluorometer an excitation source configured to generate an excitation beam, a fluorescence detector configured to a fluorescence beam, and a housing configured to receive a reagent kit for detecting bilirubin in a fluid sample. The reagent kit includes a body defining at least one fluid receiving well and an optical window positioned over each at least one fluid receiving well and a light passage window opposite each optical window. Each window is formed of a material having a fluorescence intensity that is of a lower magnitude than the fluorescence to be detected from the bilirubin. A light sensor within the housing is configured to detect light passing through the reagent kit.

Claims

1. A hematofluorometer comprising:

an excitation source configured to generate an excitation beam,

a fluorescence detector configured to a fluorescence beam;

a housing configured to receive a reagent kit reagent, the reagent kit comprising a body defining at least one fluid receiving well and an optical window positioned over each at least one fluid receiving well and a light passage window opposite each optical window, each optical window formed of a material having a fluorescence intensity that is of a lower magnitude than the fluorescence to be detected from the bilirubin, and position the reagent kit such that the excitation beam passes through one of the optical windows toward the respective well and the reflected fluorescence beam passes through the same optical window and is detected by the fluorescence detector; and

a light sensor within the housing configured to detect light passing through the reagent kit.

2. The hematofluorometer according to claim 1, wherein an intensity of the detected light is used to measure light absorbance of the fluid within the well.

3. The hematofluorometer according to claim 1, wherein an intensity of the detected light is used to validate that the excitation beam passed through the fluid in the well.

4. The hematofluorometer according to claim 1 further comprising a temperature sensor and processor wherein the processor is configured to correct for the temperature dependent response of the instrument or changes in the fluorescence of the reagents.

5. The hematofluorometer according to claim 1 further comprising a processor configured to correct for a hematocrit or hemoglobin content of the fluid.