Parsing Events During MS3 Experiments
First Claim
1. A system for reducing the time period of a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, comprising:
- a mass spectrometer that performs an MS3 experiment on a sample;
a processor in communication with the mass spectrometer thatdivides a CID event of the MS3 experiment into two time periods,at the beginning of a first time period of the CID event, instructs the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage, andat the beginning of a second time period of the CID event, instructs the mass spectrometer to both close the pulse valve and apply a second CID voltage;
wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time thereby reducing the overall time period of the CID event.
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Accused Products
Abstract
Systems and methods are provided for reducing the time period of a CID event of an MS3 experiment and making the overall fragmentation event more generic. A CID event of an MS3 experiment performed on a sample by a mass spectrometer is divided into two time periods using a processor. At the beginning of a first time period of the CID event, the mass spectrometer is instructed to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage. At the beginning of a second time period of the CID event, the mass spectrometer is instructed to both close the pulse valve and apply a second CID voltage. The mass spectrometer is pumped down during the second time period. The overlap in time of the pump down and CID reduces the overall time period of the CID event.
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Citations
20 Claims
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1. A system for reducing the time period of a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, comprising:
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a mass spectrometer that performs an MS3 experiment on a sample; a processor in communication with the mass spectrometer that divides a CID event of the MS3 experiment into two time periods, at the beginning of a first time period of the CID event, instructs the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage, and at the beginning of a second time period of the CID event, instructs the mass spectrometer to both close the pulse valve and apply a second CID voltage;
wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time thereby reducing the overall time period of the CID event. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for reducing the time period of a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, comprising:
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dividing a CID event of an MS3 experiment performed on a sample by a mass spectrometer into two time periods using a processor; at the beginning of a first time period of the CID event, instructing the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage using the processor; and at the beginning of a second time period of the CID event, instructing the mass spectrometer to both close the pulse valve and apply a second CID voltage using the processor;
wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time thereby reducing the overall time period of the CID event. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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15. A computer program product, comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for reducing the time period of a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, the method comprising:
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providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise an analysis module and a control module; dividing a CID event of an MS3 experiment performed on a sample by a mass spectrometer into two time periods using the analysis module; at the beginning of a first time period of the CID event, instructing the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage using the control module; and at the beginning of a second time period of the CID event, instructing the mass spectrometer to both close the pulse valve and apply a second CID voltage using the control module;
wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time thereby reducing the overall time period of the CID event. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification