Method for Producing Plant Seed Containing Endophytic Micro-Organisms
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Abstract
The invention discloses a method for producing plant seed containing endophytic micro-organisms characterised in by the following steps: —contacting a flowering plant with a preparation of endophytic microorganisms, whereby the endophytic microorganisms enter the plant via the flowers and are conveyed to seed produced by the plant; and—obtaining the plant seed containing endophytic microorganisms from the plant.
54 Citations
47 Claims
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1-27. -27. (canceled)
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28. A plant seed population produced by the process of i) providing an agriculturally-acceptable preparation comprising a population of isolated bacterial endophytes;
- ii) specifically contacting at least one flower of a flowering plant with the preparation under conditions such that the bacterial endophytes are localized to a plurality of seeds derived from the flowering plant, wherein the plurality of seeds individually contain at least about 100 bacterial endophytes per seed; and
iii) collecting the plurality of derived seeds, thereby producing a plant seed population. - View Dependent Claims (29, 30, 31, 32, 33, 34)
- ii) specifically contacting at least one flower of a flowering plant with the preparation under conditions such that the bacterial endophytes are localized to a plurality of seeds derived from the flowering plant, wherein the plurality of seeds individually contain at least about 100 bacterial endophytes per seed; and
- 35. A method for producing a synthetic combination comprising a plant seed and endophytic microorganisms, comprising contacting at least one flower of a flowering plant with a preparation comprising a purified population of microorganisms, the population comprising an endophytic microorganism, wherein the endophytic microorganisms are present in the interior of the plant seed.
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44. A method of improving a trait in a seed or plant produced by the seed comprising:
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contacting at least one flower of a flowering plant with a preparation comprising a purified population of microorganisms, the population comprising an endophytic microorganism, and obtaining the seed produced by the plant. - View Dependent Claims (45, 46)
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Specification