STEM CELL-IMPREGNATED THERAPEUTIC PATCH

• US 20150335788A1
• Filed: 03/05/2015
• Published: 11/26/2015
• Est. Priority Date: 05/22/2014
• Status: Active Application

• Current Assignee: Johns Hopkins University
• Original Assignee: Johns Hopkins University
• Inventors: Xia, Zhiyong, Hwang, Chao-Wei, Calderon-Colon, Xiomara, Johnston, Peter V., Bogdan, Virginia E.
• Application Number:
  US14/639,162
• Publication Number:
  US 20150335788A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61K 9/7007 Drug-containing films, membranes or sheets A61K9/0041, A61K9/0043, A61K9/006, A61K9/0063 take precedence
• CPC Class Codes: A61L 2300/412 Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2400/12 Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
• CPC Class Codes: A61L 2430/34 for soft tissue reconstruction
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body

Abstract

A therapeutic patch includes at least one layer of a nanofiber fabric and at least one of a plurality of stem cells or a plurality of stem cell-derived paracrine factors embedded in the nanofiber fabric. The therapeutic patch is produced such that the nanofiber fabric is formed of a nanofiber web.

Claims

1. A therapeutic patch, comprising:

at least one layer of a nanofiber fabric; and

at least one of a plurality of stem cells and a plurality of stem cell-derived paracrine factors embedded in the nanofiber fabric,

wherein the nanofiber fabric comprises a nanofiber web.

2. The therapeutic patch according to claim 1, wherein the nanofiber fabric comprises a nonwoven nanofiber fabric having a fiber diameter from about 80 nm to about 2 μ

m.

3. The therapeutic patch according to claim 1, wherein the nanofiber fabric comprises a plurality of pores, said plurality of pores comprising a maximal pore size.

4. The therapeutic patch according to claim 3, wherein the embedded stem cells comprise an embedded stem cell diameter, and

wherein the maximal pore size is less than the embedded stem cell diameter, the maximal pore size is less than other cell diameters, and the maximal pore size is greater than embedded paracrine factor, cell product, nutrient, or waste diameters.

5. The therapeutic patch according to claim 1, wherein the nanofiber fabric comprises a biodegradable polymer.

6. The therapeutic patch according to claim 4, wherein the nanofiber fabric comprises at least one of a synthetic biocompatible material, a biological material, and any combination thereof.

7. The therapeutic patch according to claim 5, wherein the nanofiber fabric comprises at least one of poly(L-lactic acid), poly(lactic-co-glycolic acid), polycaprolactone, polyethylene oxide, poly(ethylene terephthalate), poly(vinyl alcohol), collagen, and any combination thereof.

8. A method of treatment, comprising:

preparing a therapeutic patch comprising;

at least one layer of a nanofiber fabric; and

at least one of a plurality of stem cells or a plurality of stem cell-derived paracrine factors embedded in the nanofiber fabric; and

applying the therapeutic patch to an object,

wherein the nanofiber fabric comprises a nanofiber web.

9. The method of treatment according to claim 8, wherein the nanofiber fabric comprises a nonwoven nanofiber fabric having a fiber diameter from about 80 nm to about 2 μ

m.

10. The method of treatment according to claim 8, wherein the nanofiber fabric comprises a plurality of pores, said plurality of pores comprising a maximal pore size.

11. The method of treatment according to claim 10, wherein the embedded stem cells comprise an embedded stem cell diameter, and

wherein the maximal pore size is less than the embedded stem cell diameter, the maximal pore size is less than other cell diameters, and the maximal pore size is greater than embedded paracrine factor, cell product, nutrient, or waste diameters.

12. The method of treatment according to claim 8, wherein the nanofiber fabric comprises a biodegradable polymer.

13. The method of treatment according to claim 11, wherein the nanofiber fabric comprises at least one synthetic biocompatible material, a biological material, and any combination thereof.

14. The method of treatment according to claim 12, wherein the nanofiber fabric comprises at least one of poly(L-lactic acid), poly(lactic-co-glycolic acid) copolymer, polycaprolactone, poly(ethylene oxide), poly(ethylene terephthalate), poly(vinyl alcohol), collagen, and any combination thereof.

15. The method of treatment according to claim 8, wherein applying the therapeutic patch to an object comprises applying the therapeutic patch to at least one of a tissue, an organ or portion of an organ, a blood vessel, a muscle, a bone, a joint, a bypass graft, a medical device, and any combination thereof.

16. The method of treatment according to claim 15, wherein applying the therapeutic patch to an object comprises applying the therapeutic patch to a stent, a pacemaker, an implantable cardioverter-defibrillator, a pacemaker lead, an implantable cardioverter-defibrillator lead, a biventricular implantable cardioverter-defibrillator lead, an artificial heart, an artificial valve, a ventricular assist device, a balloon pump, a catheter, a central venous line, an orthopedic implant, wound healing packing, and wound healing gauze.

17. The method of treatment according to claim 8, wherein preparing a therapeutic patch comprises preparing a single-use therapeutic patch including stem cell-derived paracrine factors.

18. The method of treatment according to claim 17, wherein preparing the single-use therapeutic patch comprises at least one of directly absorbing conditioned media into the therapeutic patch, immersing the therapeutic patch in a homogenized cell solution, and lysing stem cells in the therapeutic patch.

19. The method of treatment according to claim 8, wherein preparing a therapeutic patch comprises preparing a reusable therapeutic patch including stem cells.

20. The method of treatment according to claim 19, wherein preparing the reusable therapeutic patch comprises:

seeding stem cells into the therapeutic patch; and

incubating the stem cells in the therapeutic patch.