CREATION OF LIBRARIES OF DROPLETS AND RELATED SPECIES
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Abstract
The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
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Citations
122 Claims
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1-120. -120. (canceled)
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121. A method, comprising:
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providing a plurality of droplets, each of the plurality of droplets comprising a first fluid and being surrounded by a second fluid, wherein the first fluid and the second fluid are substantially immiscible; causing at least some of the plurality of microfluidic droplets to undergo a phase change to form a plurality of rigidified droplets; separating the plurality of rigidified fluidic droplets into at least a first group of rigidified droplets and a second group of rigidified droplets; adding at least one first species to at least some of the rigidified droplets of the first group and at least one second species to at least some of the rigidified droplets from the second group, wherein the second species is distinguishable from the first species; forming a suspension comprising at least one rigidified droplet from each of the groups of rigidified droplets; Wherein the rigidified droplets are capable of fluidization.
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122-155. -155. (canceled)
Specification