CREATION OF LIBRARIES OF DROPLETS AND RELATED SPECIES

• US 20150337371A1
• Filed: 07/29/2015
• Published: 11/26/2015
• Est. Priority Date: 09/19/2008
• Status: Abandoned Application

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Weitz, David A., Agresti, Jeremy
• Application Number:
  US14/812,954
• Publication Number:
  US 20150337371A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B01J 13/04 by physical processes, e.g. drying, spraying
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00709 Type of synthesis
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00716 Heat activated synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.

Claims

1-91. -91. (canceled)

92. A composition, comprising:

a container comprising at least eight mutually distinguishable rigidified droplets, each having substantially the same composition but containing a distinguishable species with respect to the other rigidified droplets.

99. The composition of claim 92, wherein the composition comprises a gel.

105. The composition of claim 99, wherein the gel may be fluidized.

106. The composition of claim 92, wherein the composition comprises a polymer.

110. The composition of claim 106, wherein the polymer may be fluidized.

111-155. -155. (canceled)