Differentiation of Human Embryonic Stem Cells into Single Hormonal Insulin Positive Cells
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Abstract
The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides methods to produce a population of cells, wherein greater than 10% of the cells in the population express markers characteristic of single hormonal pancreatic beta cells.
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Citations
25 Claims
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1. (canceled)
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2. (canceled)
- 3. An invitro differentiated population of pancreatic endoderm cells obtained from the stepwise differentiation of pluripotent cells, wherein cells at each step of differentiation are cultured in medium comprising 5 mM to 20 mM glucose, and wherein greater than 10% of the cells in the differentiated population are single hormonal insulin positive cells.
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10-23. -23. (canceled)
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