DETECTION OF DNA OR RNA USING SINGLE MOLECULE ARRAYS AND OTHER TECHNIQUES
First Claim
1. A method for determining detecting or measuring the concentration of DNA or RNA molecules in a sample, comprising:
- fragmenting DNA or RNA from the sample to form fragmented DNA or RNA sequences;
exposing the fragmented DNA or RNA sequences to at least a first type of binding ligand and a second type of binding ligand, wherein at least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand comprises a first nucleic acid sequence complimentary to a first portion of a sequence contained in the DNA or RNA and second type of binding ligand comprises a second nucleic acid sequence complimentary to a second portion of a sequence contained in the DNA or RNA, wherein the first nucleic acid sequence and the second nucleic acid sequence are different; and
analyzing a portion of the sample from the exposing step to determine the number of fragmented DNA or RNA sequences associated at least one of the first type of binding ligand and the second type of binding ligand, wherein the first type of binding ligand and the second type of binding ligand produce substantially similar signals.
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Accused Products
Abstract
Described herein are methods and systems for detecting DNA or RNA using single molecules array or other techniques. DNA or RNA from the sample may be fragmented and exposed to a first type of binding ligand and a second type of binding ligand that comprise nucleic acid sequences complimentary at least a portion of a sequence contained in the target DNA or RNA. At least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand and second type of binding ligand comprises nucleic acid sequences complimentary to a different portions of a sequence contained in the DNA or RNA. A portion of the sample exposed to the binding ligands is analyzed to determine the number of fragmented DNA or RNA sequences.
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Citations
58 Claims
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1. A method for determining detecting or measuring the concentration of DNA or RNA molecules in a sample, comprising:
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fragmenting DNA or RNA from the sample to form fragmented DNA or RNA sequences; exposing the fragmented DNA or RNA sequences to at least a first type of binding ligand and a second type of binding ligand, wherein at least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand comprises a first nucleic acid sequence complimentary to a first portion of a sequence contained in the DNA or RNA and second type of binding ligand comprises a second nucleic acid sequence complimentary to a second portion of a sequence contained in the DNA or RNA, wherein the first nucleic acid sequence and the second nucleic acid sequence are different; and analyzing a portion of the sample from the exposing step to determine the number of fragmented DNA or RNA sequences associated at least one of the first type of binding ligand and the second type of binding ligand, wherein the first type of binding ligand and the second type of binding ligand produce substantially similar signals. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method for determining detecting or measuring the concentration of DNA or RNA molecules in sample, comprising:
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fragmenting DNA or RNA from a sample to form fragmented DNA or RNA sequences; exposing the fragmented DNA or RNA sequences to at least a first type of capture object and a second type of capture object, wherein at least a portion of the fragmented DNA or RNA associates with the least one of the first type of capture object and the second type of capture objection object, wherein the first type of capture object comprises a first capture probe comprising a first nucleic acid sequence complimentary to a portion of a sequence contained in the DNA or RNA and the second type of capture object comprises a second capture probe comprising a second nucleic acid sequence complimentary to a portion of a sequence contained in the DNA or RNA, wherein the first sequence and the second sequence are different; and analyzing a portion of the sample from the exposing step to determine the number of fragmented DNA or RNA sequences associated with at least one of the first type of capture object and the second type of capture object. - View Dependent Claims (39, 40, 41, 42, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
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43. The method of claim 43, wherein the average volume of the plurality of reaction vessels is between about 10 attoliters and about 100 picoliters, or between about 1 femtoliter and about 1 picoliter.
Specification