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Methods for Screening Proteins Using DNA Encoded Chemical Libraries as Templates for Enzyme Catalysis

  • US 20150361420A1
  • Filed: 08/31/2015
  • Published: 12/17/2015
  • Est. Priority Date: 03/21/2012
  • Status: Active Grant
First Claim
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1. A method for screening a protein library for a protein having a desired activity, the method comprising:

  • a) providing, at distinct features of a solid support, a plurality of nucleic acids, each nucleic acid having a predefined sequence;

    b) expressing in vitro a plurality of proteins from the plurality of nucleic acids;

    c) exposing the plurality of proteins to two single-stranded oligonucleotides having complementary sequences, each single-stranded oligonucleotide having a reactant and a fluorophore;

    d) detecting fluorescence of a complex having a protein having the desired activity, the reactant, the single-stranded oligonucleotide and the fluorophore; and

    e) isolating the complex showing detectable fluorescence energy transfer, thereby isolating the protein having the desired activity.

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