GENETICALLY MODIFIED BACTERIA AND METHODS FOR GENETIC MODIFICATION OF BACTERIA
First Claim
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1. A genetically modified bacteria of the genus Propionibacterium comprising a nucleic acid encoding a mammalian growth factor or a mammalian cytokine.
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Abstract
Microbes can be genetically modified to express biomolecules that are beneficial to mammals and/or to reduce, or eliminate, expression of harmful virulence factors. The growth and viability of such genetically modified microbes can optionally be controlled by inducible promoters that regulate the expression of proteins that are essential to their growth and survival. Compositions comprising such genetically modified microbes as well as methods of making and using the same are disclosed herein.
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52 Claims
- 1. A genetically modified bacteria of the genus Propionibacterium comprising a nucleic acid encoding a mammalian growth factor or a mammalian cytokine.
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44. A genetically modified bacteria of the species Propionibacterium acnes comprising:
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a) a mammalian growth factor, wherein the mammalian growth factor is secreted; and b) an inducible promoter directing the expression of an essential protein selected from the group consisting of a chromosome replication initiator protein DnaA, FtsA, FtsI, FtsL, FtsK, FtsN, FtsQ, FtsW, FtsZ, ZipA, aroE, atpD, gmk, guaA, lepA, recA, and sodA; wherein the expression of an endogenous CAMP2 and/or endogenous GADPH protein is substantially reduced or eliminated in the genetically modified bacteria. - View Dependent Claims (46)
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45. A genetically modified bacteria of the species Propionibacterium acnes comprising:
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a) a human IL10, wherein the IL10 is secreted; and b) an inducible promoter directing the expression of an essential protein selected from the group consisting of a chromosome replication initiator protein DnaA, FtsA, FtsI, FtsL, FtsK, FtsN, FtsQ, FtsW, FtsZ, ZipA, aroE, atpD, gmk, guaA, lepA, recA, and sodA; wherein the expression of an endogenous CAMP2 and/or endogenous GADPH protein is substantially reduced or eliminated in the genetically modified bacteria.
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Specification