Systems and Methods for Single Cell Culture and Analysis by Microscopy and Maldi Mass Spectrometry
First Claim
1. An isolated cell analysis device comprising a substrate having a first surface comprising a plurality of wells,the first surface comprising the plurality of wells configured to receive a suspension comprising a plurality of cells,the plurality of wells configured to have at least 25% of the wells contain one and only one cell upon receiving the suspension comprising a plurality of cells, waiting a length of time to allow the plurality of cells to settle, and removing an excess of the suspension from the first surface,each of the plurality of wells configured to allow an isolated cell contained within one of the plurality of wells to be optically interrogated and to subsequently have molecules of the isolated cell be ionized by matrix assisted laser desorption ionization via a laser impinging on the isolated cell at an angle between 5 and 85 degrees relative to the first surface.
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Accused Products
Abstract
Systems and methods for single cell culture and analysis by microscopy and matrix assisted laser desorption ionization mass spectrometry are disclosed. The systems and methods isolate a plurality of cells in a plurality of wells such that a predetermined number of the plurality of wells contain one and only one cell. The plurality of wells allow for optical interrogation of the cells and subsequent matrix assisted laser desorption ionizing of molecules within the cells.
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Citations
20 Claims
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1. An isolated cell analysis device comprising a substrate having a first surface comprising a plurality of wells,
the first surface comprising the plurality of wells configured to receive a suspension comprising a plurality of cells, the plurality of wells configured to have at least 25% of the wells contain one and only one cell upon receiving the suspension comprising a plurality of cells, waiting a length of time to allow the plurality of cells to settle, and removing an excess of the suspension from the first surface, each of the plurality of wells configured to allow an isolated cell contained within one of the plurality of wells to be optically interrogated and to subsequently have molecules of the isolated cell be ionized by matrix assisted laser desorption ionization via a laser impinging on the isolated cell at an angle between 5 and 85 degrees relative to the first surface.
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12. A method of making a single cell analysis device, the method comprising:
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forming a pattern of a plurality of wells in a substrate; and applying a MALDI-suitable material to at least a portion of each of the plurality of wells in the substrate. - View Dependent Claims (13)
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14. A method of optically interrogating and subsequently matrix assisted laser desorption ionizing a plurality of isolated cells, the method comprising:
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isolating a plurality of cells in a plurality of wells, such that at least 25% of the plurality of wells contain one and only one cell of the plurality of cells; optically interrogating at least the plurality of isolated cells located in the plurality of wells containing one and only one cell; introducing a matrix assisted laser desorption ionization matrix into the plurality of wells; and matrix assisted laser desorption ionizing the at least one cell. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification