PROTEINS WITH MODIFIED GLYCOSYLATION AND METHODS OF PRODUCTION THEREOF
First Claim
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1. A method of producing protein with modified glycosylation, the method comprising:
- providing a transgenic mammal that has been modified to have increased expression of sialyl transferase in the mammary gland,wherein the transgenic mammal expresses a protein in the mammary gland, andharvesting the protein from the mammary gland of the transgenic mammal.
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Abstract
In one aspect, the disclosure provides proteins with modified glycosylation and methods of their production. In aspect, the disclosure provides transgenic animals and cells for the production of proteins with modified glycosylation. In some embodiment, the modified glycosylation is increased sialylation.
4 Citations
38 Claims
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1. A method of producing protein with modified glycosylation, the method comprising:
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providing a transgenic mammal that has been modified to have increased expression of sialyl transferase in the mammary gland, wherein the transgenic mammal expresses a protein in the mammary gland, and harvesting the protein from the mammary gland of the transgenic mammal. - View Dependent Claims (3, 4, 6, 7, 8, 9, 10, 11, 12, 19, 20, 33, 34, 35, 36, 37, 38)
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2. A method of producing protein with modified glycosylation, the method comprising:
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providing a mammary epithelial cell that has been modified to have increased expression of sialyl transferase, wherein the mammary epithelial cell expresses a protein, and harvesting the protein from the mammary epithelial cells.
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5. A method of producing protein with increased sialylation, the method comprising:
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providing a transgenic mammal that has been modified to have increased expression of sialyl transferase in the mammary gland, wherein the transgenic mammal expresses a protein in the mammary gland, and harvesting the protein with increased sialylation from the mammary gland of the transgenic mammal.
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13. A method of increasing the sialyl transferase activity in the mammary gland, the method comprising:
providing a transgenic mammal that has been modified to have increased expression of sialyl transferase in the mammary gland.
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14. A method of producing sialyl transferase, the method comprising:
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providing a transgenic mammal that has been modified to have increased expression of sialyl transferase in the mammary gland, and harvesting the sialyl transferase from the mammary gland of the transgenic mammal. - View Dependent Claims (15, 16, 17, 18)
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- 21. A transgenic mammal that has been modified to have increased expression of sialyl transferase in the mammary gland.
- 27. A mammary epithelial cell that has been modified to have increased expression of sialyl transferase.
Specification