BIOSENSOR INCORPORATING PROTEIN-IMMOBILIZED MEMBRANE AND METHOD OF IMMOBILIZING PROTEIN IN BIOSENSOR
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Abstract
The present invention relates to a protein-immobilized membrane (14) including a cell membrane homologous layer (14A) and a protein (14B) immobilized to the cell membrane homologous layer (14A), where the protein contains cytochrome or a cytochrome complex. The present invention also relates to a method for forming a protein-immobilized membrane (14), and an enzyme-immobilized electrode and a biosensor (X1) provided with a protein-immobilized membrane (14). Preferably, the cell membrane homologous layer (14A) may contain a phospholipid polymer, and the protein (14B) may be CyGDH including an α subunit having a glucose dehydrogenase activity and cytochrome C having a function of electron transfer.
2 Citations
44 Claims
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1-30. -30. (canceled)
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31. A biosensor, comprising:
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a substrate; a capillary formed on the substrate for passage of a fluid to be analyzed; an electrode formed on the substrate; and a reagent portion formed on the substrate to cover the electrode; wherein the reagent portion comprises; an orientation-controlling layer formed on the electrode in contact therewith; and a layer of protein that is immobilized on a side of the orientation-controlling layer opposite the electrode in direct contact with the orientation-controlling layer, the protein containing a cytochrome or a cytochrome complex, and the cytochrome or cytochrome complex of the protein being oriented toward the orientation-controlling layer. - View Dependent Claims (32, 33, 34, 35, 36)
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37. A method for immobilizing a protein in a biosensor, the biosensor comprising:
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a substrate; a capillary formed on the substrate for passage of a fluid to be analyzed; and an electrode formed on the substrate; the method comprising; forming an orientation-controlling layer on a portion of the electrode in contact therewith; and causing a layer of protein to immobilize on a side of the orientation-controlling layer opposite the electrode in direct contact with the orientation-controlling layer, the protein containing cytochrome or a cytochrome complex, the cytochrome or cytochrome complex of the protein being oriented toward the orientation-controlling layer. - View Dependent Claims (38, 39, 40, 41, 42, 43, 44)
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Specification