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METHODS OF UPSCALING MESENCHYMAL STROMAL CELL PRODUCTION, COMPOSITIONS AND KIT THEREOF

  • US 20160002601A1
  • Filed: 01/14/2015
  • Published: 01/07/2016
  • Est. Priority Date: 03/03/2011
  • Status: Abandoned Application
First Claim
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1. A process for culturing of mesenchymal stromal cells, said process comprising act of:

  • a) allowing the mesenchymal stromal cells cultured till a first predetermined passage, to expand to a second predetermined passage in presence of culture media comprising basic fibroblast growth factor (bFGF);

    wherein said expansion is carried out by contacting the cells with said culture media when said cells achieve at least one pre-determined confluency; and

    wherein the process increases number of the cells at the end of the second predetermined passage by 2000 folds when compared to number of the cells at the first predetermined passage.

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