METHODS FOR CAPTURING NUCLEIC ACIDS
First Claim
1. A method of capturing a target nucleic acid, comprising:
- applying a nucleic acid capture probe to a capture zone of a substrate, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complimentary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate;
applying a sample comprising the target nucleic acid having a second molecular weight to a sample application zone of the substrate;
wherein the sample comprising the target nucleic acid flows across a length of the substrate from the sample application zone to the capture zone by lateral flow, and the target nucleic acid is captured by the nucleic acid capture probes by hybridization at the capture zone,wherein the nucleic acid capture probe is a rolling circle amplification (RCA) product.
3 Assignments
0 Petitions
Accused Products
Abstract
A method is provided herein, wherein the method of capturing a target nucleic acid, comprises applying a nucleic acid capture probe to a capture zone of a needs definition, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complimentary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate. The method further comprises applying a sample comprising the target nucleic acid having a second molecular weight to a sample application zone of the substrate; wherein the sample comprising the target nucleic acid flows across a length of the substrate from the sample application zone to the capture zone by lateral flow, and the target nucleic acid is captured by the nucleic acid capture probes by hybridization to the capture zone.
-
Citations
29 Claims
-
1. A method of capturing a target nucleic acid, comprising:
-
applying a nucleic acid capture probe to a capture zone of a substrate, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complimentary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate; applying a sample comprising the target nucleic acid having a second molecular weight to a sample application zone of the substrate;
wherein the sample comprising the target nucleic acid flows across a length of the substrate from the sample application zone to the capture zone by lateral flow, and the target nucleic acid is captured by the nucleic acid capture probes by hybridization at the capture zone,wherein the nucleic acid capture probe is a rolling circle amplification (RCA) product. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
-
-
28. A method of capturing target nucleic acids, comprising:
-
applying nucleic acid capture probes to a capture zone of a substrate, wherein the nucleic acid capture probes having a first molecular weight comprise at least a sequence that is complementary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probes are substantially immobilized at the capture zone of the substrate; applying a sample comprising the target nucleic acids having a second molecular weight to a sample application zone of the substrate; and flowing a liquid across a length of the substrate from the sample application zone to the capture zone, wherein the target nucleic acids migrate away from the sample application zone to the capture zone by lateral flow and are captured by the nucleic acid capture probes by hybridization.
-
-
29. A method of capturing target nucleic acids, comprising:
-
applying a nucleic acid capture probe to a capture zone of a substrate, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complementary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate; applying a sample comprising one or more of the target nucleic acid having a second molecular weight, a template nucleic acid having a third molecular weight to a sample application zone of a substrate; flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to the capture zone, wherein the template nucleic acids are amplified to form the target nucleic acids; separating the target nucleic acids from the template nucleic acids by lateral flow, wherein the target nucleic acids migrate away from the sample application zone to the capture zone and are captured by the nucleic acid capture probes by hybridization without a washing step.
-
Specification