NOVEL POLYPEPTIDE EXHIBITING FLUORESCENT PROPERTIES AND USE THEREOF
First Claim
1. A polypeptide shown in any of the following (1) to (4) and having fluorescent properties in the presence of bilirubin:
- (1) a polypeptide including the amino acid sequence of SEQ ID NO;
1;
(2) a polypeptide including an amino acid sequence having substitution, deletion, insertion, and/or addition of 1 to 21 amino acids in the amino acid sequence of SEQ ID NO;
1;
(3) a polypeptide having 85% or more sequence identity to the amino acid sequence of SEQ ID NO;
1; and
(4) a polypeptide including an amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of the sequence complementary to a polynucleotide encoding the polypeptide according to the above (1) under a stringent condition.
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Accused Products
Abstract
In order to provide a novel fluorescent protein and use thereof, the polypeptide according to the present invention has fluorescent properties in the presence of bilirubin and includes (1) the amino acid sequence of SEQ ID NO: 1, (2) an amino acid sequence having, for example, substitution of 1 to 21 amine acids in the amino acid sequence of SEQ ID NO: 1, (3) an amino acid sequence having 85% or more sequence identity to the amino acid sequence of SEQ ID NO: 1, or (4) the amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of a sequence complementary to the polynucleotide encoding the polypeptide according to the amino acid sequence in (1) under a stringent condition.
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Citations
18 Claims
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1. A polypeptide shown in any of the following (1) to (4) and having fluorescent properties in the presence of bilirubin:
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(1) a polypeptide including the amino acid sequence of SEQ ID NO;
1;(2) a polypeptide including an amino acid sequence having substitution, deletion, insertion, and/or addition of 1 to 21 amino acids in the amino acid sequence of SEQ ID NO;
1;(3) a polypeptide having 85% or more sequence identity to the amino acid sequence of SEQ ID NO;
1; and(4) a polypeptide including an amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of the sequence complementary to a polynucleotide encoding the polypeptide according to the above (1) under a stringent condition. - View Dependent Claims (3, 6, 7, 8, 9, 10, 11, 13, 15, 18)
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2. A polynucleotide shown in any of the following (1) to (4):
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(1) a polynucleotide encoding a polypeptide including the amino acid sequence of SEQ ID NO;
1;(2) a polynucleotide encoding a polypeptide including an amino acid sequence having substitution, deletion, insertion, and/or addition of 1 to 21 amino acids in the amino acid sequence of SEQ ID NO;
1 and having fluorescent properties in the presence of bilirubin;(3) a polynucleotide encoding a polypeptide having 85% or more sequence identity to the amino acid sequence of SEQ ID NO;
1 and having fluorescent properties in the presence of bilirubin; and(4) a polynucleotide hybridizing with a polynucleotide consisting of a sequence complementary to the polynucleotide according to the above (1) under a stringent condition and encoding a polypeptide having fluorescent properties in the presence of bilirubin. - View Dependent Claims (4, 5, 12, 14, 16, 17)
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Specification