METHOD OF MANUFACTURING A DEVICE FOR SUPPORTING BIOLOGICAL MATERIAL GROWTH AND DEVICE THEREFROM

• US 20160010045A1
• Filed: 02/25/2014
• Published: 01/14/2016
• Est. Priority Date: 02/28/2013
• Status: Abandoned Application

• Current Assignee: Nanyang Technology University, National University of Singapore
• Original Assignee: Nanyang Technology University, National University of Singapore
• Inventors: COHEN, Yehuda, SHEARWOOD, Christopher, PRAJAPATI, Vishal
• Application Number:
  US14/771,434
• Publication Number:
  US 20160010045A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B29C 45/006 Joining parts moulded in separate cavities
• CPC Class Codes: B29K 2069/00 Use of PC, i.e. polycarbonates or derivatives thereof, as moulding material
• CPC Class Codes: B29K 2995/0037 Other properties
• CPC Class Codes: B29L 2009/00 Layered products
• CPC Class Codes: B29L 2031/712 Containers; Packaging elements or accessories, Packages closures therefor B29L2031/56; ink or toner cartridges B29L2031/7678; squeeze tubes B29L2023/20; suitcases B29L2031/7418
• CPC Class Codes: C12M 23/04 Flat or tray type, drawers C12M23/10, C12M23/12, C12M23/16 take precedence
• CPC Class Codes: C12M 23/22 Transparent or translucent parts glassware for laboratory use B01L3/00
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 41/34 of gas
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404

Abstract

Provided is a method of manufacturing a device for supporting biological material growth, including forming a first platform layer through a molding process, the first base layer including a central chamber; and a plurality of elongate channels coupling the central chamber to the periphery of the first platform layer; forming a second platform layer through a molding process; and coupling the first platform layer to the second platform layer. Accordingly, a device for supporting biological material growth is also provided.

Claims

1. A method of manufacturing a device for supporting biological material growth, comprising:

forming a first platform layer through a molding process, the first platform layer comprising;

a central chamber; and

a plurality of elongate channels coupling the central chamber to the periphery of the first platform layer;

forming a second platform layer through a molding process; and

coupling the first platform layer to the second platform layer.

2. The method of claim 1, wherein the coupling of the first platform layer to the second platform layer is an airtight and permanent coupling.

3. The method of claim 2, wherein the coupling of the first platform layer to the second platform layer is any one of chemical welding, ultrasonic welding, laser welding, indium welding, or adhesion.

4. The method of claim 1, wherein the molding process is any one of injection molding, split-injection molding, powder injection molding, casting or machining.

5. The method of claim 1, wherein the first platform layer and the second platform layer comprise any one of thermoplastic, thermoset, copolymers, ceramic glass, or metallic materials.

6. The method of claim 1, further comprising:

coupling a removable cover to any one of the first platform layer and the second platform layer, the removable cover comprising an observation portion arranged for a viewing of the central chamber.

7. The method of claim 6, wherein the coupling of the removable cover is a snap-fit coupling.

8. The method of claim 6, further comprising:

utilizing a glass cover slip and a elastomeric gasket to provide the observation portion of the removable cover.

9. The method of claim 1, further comprising:

treating the channels for preventing biological material growth in the elongate channels, comprising any one of micro-embossing the elongate channels, coating the elongate channel with a ceramic overlay, coating the elongate channel with a polymer overlay, or coating the elongate channel with a metallic overlay.

10. The method of claim 1, further comprising sandwiching a metallic layer between the first platform layer and the second platform layer prior to coupling the first platform layer to the second platform layer.

11. A device for supporting biological material growth, comprising:

a first platform layer, formed through a molding process, the first platform layer comprising;

a central chamber; and

a plurality of elongate channels coupling the central chamber to the periphery of the first platform layer; and

a second platform layer, formed through a molding process;

wherein the first platform layer is coupled to the second platform layer to form a growth platform.

12. The device of claim 11, wherein the growth platform comprises a plurality of peripheral apertures at the periphery of the growth platform, the peripheral apertures coupled to the central chamber through the elongate channels.

13. The device of claim 12, wherein the growth platform comprises four peripheral apertures, each of the peripheral apertures provided perpendicular to each other.

14. The device of claim 13, further comprising a branch arrangement of channels between any one of the peripheral apertures and the central chamber.

15. The device of claim 14, wherein the branch arrangement of channels comprises a channel leading from the peripheral aperture branching to form a plurality of channels leading to the central chamber.

16. The device of claim 15, wherein the channel leading from the peripheral aperture is branched twice to form the plurality of channels leading to the central chamber.

17. The device of claim 11, wherein the peripheral aperture is an extruded aperture.

18. The device of claim 11, further comprising a sensor within the device between the first platform layer and the second platform layer.

19. The device of claim 18, wherein the sensor is arranged to monitor growth of the biological material and is any one of a volumetric oxygen sensor, a plant optrode, an oxygen reporter, a thermocouple, magnetic fields sensors, high permeability structures, and electrical contact sensors.

20. The device of claim 11, further comprising an optical waveguide formed in the growth platform.

21. The device of claim 11, further comprising a Faraday cage structure formed in the device.

22. The device of claim 11, further comprising an inoculation port provided at a periphery of the growth platform.

23. The device of claim 22, wherein the inoculation port is coupled to the chamber by an inoculation port channel, the inoculation port channel separate from any fluid-carrying channel in the device.