METHODS OF DETECTING HIGH ANTIOXIDANT LEVELS DURING ELECTROCHEMICAL MEASUREMENTS AND FAILSAFING AN ANALYTE CONCENTRATION THEREFROM AS WELL AS DEVICES, APPARATUSES AND SYSTEMS INCORPORTING THE SAME
First Claim
1. A method of electrochemically measuring an analyte in a fluid sample in the presence of an antioxidant, the method comprising the steps of:
- applying an electrical test sequence to an electrochemical biosensor, the biosensor comprising;
an electrode system,a reagent including a redox mediator in electrical communication with the electrode system, anda receptacle configured to contact the fluid sample provided to the biosensor,with the fluid sample in fluidic contact with the reagent, wherein the test sequence comprises at least one direct current (DC) block, wherein the at least one DC block includes a slow-ramp bi-polar (SRBP) waveform, and wherein a closed circuit condition of the electrode system is maintained during the at least one DC block;
measuring current response information to the test sequence, including information from the at least one recovery potential; and
evaluating quantitatively a level of the antioxidant present in the fluid sample based at least in part upon current response information to the SRBP waveform.
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Abstract
Methods are disclosed for measuring an analyte concentration in a fluidic sample. Such methods further allow one to provide an error code or correct and/or compensate for interferents such as an antioxidant before providing an analyte concentration. The measurement methods utilize information obtained from test sequences having at least one DC block, such as a slow-ramped bi-polar waveform, where a closed circuit condition is maintained during the DC block. The methods use information relating to status of a redox mediator feature during the electrochemical analysis to provide an antioxidant failsafe if the antioxidant is interfering with the analyte concentration. Also disclosed are devices, apparatuses and systems incorporating the various measurement methods.
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Citations
35 Claims
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1. A method of electrochemically measuring an analyte in a fluid sample in the presence of an antioxidant, the method comprising the steps of:
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applying an electrical test sequence to an electrochemical biosensor, the biosensor comprising; an electrode system, a reagent including a redox mediator in electrical communication with the electrode system, and a receptacle configured to contact the fluid sample provided to the biosensor, with the fluid sample in fluidic contact with the reagent, wherein the test sequence comprises at least one direct current (DC) block, wherein the at least one DC block includes a slow-ramp bi-polar (SRBP) waveform, and wherein a closed circuit condition of the electrode system is maintained during the at least one DC block; measuring current response information to the test sequence, including information from the at least one recovery potential; and evaluating quantitatively a level of the antioxidant present in the fluid sample based at least in part upon current response information to the SRBP waveform. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of electrochemically measuring an analyte in a fluid sample that may have one or more interferents, the method comprising the steps of:
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applying an electrical test sequence to an electrochemical biosensor, the biosensor comprising; an electrode system, a reagent including a redox mediator in electrical communication with the electrode system, and a receptacle configured to contact the fluid sample provided to the biosensor, with the fluid sample in fluidic contact with the reagent, wherein the test sequence includes a signal component configured to provide a current response varying as a function of concentration of one or more interferents in the fluid sample; and performing a reagent chemistry health failsafe check based upon an evaluation of the current response indicating the concentration of the one or more interferents. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24)
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25. A method of electrochemically measuring an analyte in a fluid sample and providing an antioxidant failsafe, the method comprising the steps of:
evaluating a level of the antioxidant present in the fluid sample based at least in part upon current response information to a slow-ramp bi-polar (SRBP) waveform applied under a closed circuit condition, wherein a failsafe is displayed if the level of the antioxidant is above a predetermined threshold. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
Specification