CD20-BINDING IMMUNOTOXINS FOR INDUCING CELLULAR INTERNALIZATION AND METHODS USING SAME
First Claim
1. A CD20-binding protein comprisinga) a CD20 binding region comprising an immunoglobulin-type binding region and capable of specifically binding an extracellular part of CD20 andb) a Shiga toxin effector polypeptide;
- whereby upon administration of the CD20-binding protein to a cell expressing CD20 on a cellular surface, the CD20-binding protein is capable of inducing rapid cellular internalization of a protein complex comprising the CD20-binding protein bound to CD20.
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Accused Products
Abstract
The present invention provides CD20-binding proteins that bind to and rapidly internalize CD20 antigens from a cell surface location to the interior of a cell. CD20-binding proteins of the invention comprise a CD20 binding region and a Shiga toxin effector region. Certain of the disclosed CD20-binding proteins kill cells that express CD20 on their surface. Further, the presently disclosed CD20-binding proteins can comprise additional exogenous materials and are capable of targeted delivery of these additional exogenous materials into the interior of CD20 expressing cells. Such additional materials may include peptides, antigens, enzymes, and polynucleotides. These CD20-binding proteins have uses in methods of internalizing themselves, targeted killing of CD20 expressing cells, delivering exogenous materials into CD20 expressing cells, and treating a variety of diseases involving CD20 expressing cells, such as cancers and immune disorders.
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Citations
46 Claims
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1. A CD20-binding protein comprising
a) a CD20 binding region comprising an immunoglobulin-type binding region and capable of specifically binding an extracellular part of CD20 and b) a Shiga toxin effector polypeptide; whereby upon administration of the CD20-binding protein to a cell expressing CD20 on a cellular surface, the CD20-binding protein is capable of inducing rapid cellular internalization of a protein complex comprising the CD20-binding protein bound to CD20. - View Dependent Claims (2, 3, 5, 6, 7, 8, 12, 13, 17, 32, 33, 36, 37, 38, 42)
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4. (canceled)
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9-11. -11. (canceled)
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18. A CD20-binding protein for the delivery of an additional exogenous material into a cell that expresses CD20 on a cellular surface, wherein the CD20-binding protein comprises
a) a CD20 binding region comprising an immunoglobulin-type polypeptide and capable of specifically binding an extracellular part of a CD20 molecule, b) a Shiga toxin effector polypeptide, and c) an additional exogenous material; whereby upon administration of the CD20-binding protein to a CD20 expressing cell, the CD20-binding protein is capable of inducing rapid cellular internalization of a protein complex comprising the CD20-binding protein bound to CD20 and capable of delivering said additional exogenous material into the interior of said cell. - View Dependent Claims (30, 44, 45)
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21-27. -27. (canceled)
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39. (canceled)
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43. (canceled)
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46-47. -47. (canceled)
Specification