PERFUSION DEVICE, CORRESPONDING APPARATUS USING SAID PERFUSION DEVICE AND METHOD TO ANALYZE THE THROMBOTIC-ISCHEMIC AND HEMORRHAGIC PATHOLOGY
First Claim
1. Perfusion device for the dynamic analysis of the thrombotic-ischemic and hemorrhagic pathology, comprising a perfusion chamber provided with at least one micro-channel configured to be connected to a feed circuit of a fluid to be analyzed, in said at least one micro-channel a reactive substrate being present, suitable to simulate a damaged vasal surface and to reproduce hemostasis phenomena and processes, said perfusion chamber being made of a material which allows the optical acquisition, in fluorescence light and/or visible light, of images or videos of the flow of fluid inside said at least one micro-channel, wherein said perfusion chamber is defined by a cartridge coupled to a covering plate between which at least one micro-groove, in the micrometrical size range, is provided, said at least one micro-groove being made open on at least one of either said cartridge or said covering plate in order to define said at least one micro-channel;
- wherein said cartridge comprises, integrated therewith in a single body;
a plurality of containing elements connected to said at least one micro-channel and configured to contain a biological fluid to be analyzed and/or one or more auxiliary fluids;
a selection valve configured to selectively put in fluidic communication one or the other of the containing elements with said at least one micro-channel;
a suction pump device located downstream of said at least one micro-channel and configured to aspirate the flow of fluid through said at least one micro-channel in controlled and selectively variable fluid-dynamic conditions.
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Accused Products
Abstract
Perfusion device for the dynamic analysis of the thrombotic-ischemic and hemorrhagic pathology, comprising at least one micro-channel (54) able to be connected to a circuit (13) and in which a fluid is able to flow, such as a biological fluid, like blood or other hematic fluids, whether they are animal or human fluids and mixtures of said fluids with additive substances, or a non-biological fluid, and in which at least one reactive substrate is present, intended for the analysis to be carried out, such as a cytoadhesive substrate, in order to simulate a damaged vasal surface and to reproduce hemostasis phenomena and processes. The perfusion chamber is made of a material which allows the optical acquisition in fluorescence light and/or in visible light of images or videos of the flow of fluid inside the at least one micro-channel (54).
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Citations
21 Claims
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1. Perfusion device for the dynamic analysis of the thrombotic-ischemic and hemorrhagic pathology, comprising a perfusion chamber provided with at least one micro-channel configured to be connected to a feed circuit of a fluid to be analyzed, in said at least one micro-channel a reactive substrate being present, suitable to simulate a damaged vasal surface and to reproduce hemostasis phenomena and processes, said perfusion chamber being made of a material which allows the optical acquisition, in fluorescence light and/or visible light, of images or videos of the flow of fluid inside said at least one micro-channel, wherein said perfusion chamber is defined by a cartridge coupled to a covering plate between which at least one micro-groove, in the micrometrical size range, is provided, said at least one micro-groove being made open on at least one of either said cartridge or said covering plate in order to define said at least one micro-channel;
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wherein said cartridge comprises, integrated therewith in a single body; a plurality of containing elements connected to said at least one micro-channel and configured to contain a biological fluid to be analyzed and/or one or more auxiliary fluids; a selection valve configured to selectively put in fluidic communication one or the other of the containing elements with said at least one micro-channel; a suction pump device located downstream of said at least one micro-channel and configured to aspirate the flow of fluid through said at least one micro-channel in controlled and selectively variable fluid-dynamic conditions. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. Method for the dynamic analysis of the thrombotic-ischemic and hemorrhagic pathology, comprising a first step in which a fluid such as a biological fluid, such as blood or other hematic fluids, whether they are animal or human fluids and mixtures of said fluids with additive substances, or a non-biological fluid, is made to flow in controlled and variable fluid-dynamic conditions through a circuit by a suction pump device, said fluid being made to pass through at least one micro-channel of a perfusion chamber in which a reactive substrate is present, intended for the analysis to be carried out, such as a cytoadhesive substrate, in order to simulate a damaged vasal surface and to reproduce hemostasis phenomena and processes, and at least one second step of optical acquisition and processing of images or videos acquired, from said at least one micro-channel, by optical acquisition means of images or videos, and processed by electronic processing means, and in which said perfusion chamber is made of a material which allows the optical acquisition, in fluorescence light and/or visible light, of images or videos of the flow of fluid inside said at least one micro-channel by means of said optical acquisition means of images or videos, wherein during said first step said fluid is introduced into said perfusion chamber which is defined by at least one cartridge coupled to a covering plate between which at least one micro-groove is provided to define said micro-channel, said micro-groove being made open on at least one of either said cartridge or said covering plate, with a desired depth, in the micrometrical size range, wherein said cartridge comprises, integrated therewith in a single body:
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a plurality of containing elements connected to said at least one micro-channel and configured to contain a biological fluid to be analyzed and/or one or more auxiliary fluids; a selection valve configured to selectively put in fluidic communication one or the other of the containing elements with said at least one micro-channel; a suction pump device, located downstream of said at least one micro-channel. - View Dependent Claims (19, 20, 21)
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Specification