Milling Process

• US 20160024228A1
• Filed: 03/04/2014
• Published: 01/28/2016
• Est. Priority Date: 03/05/2013
• Status: Abandoned Application

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Han, Wang, McLaughlin, Scott R., Long, Zhen
• Application Number:
  US14/773,589
• Publication Number:
  US 20160024228A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C08B 30/044 from corn or maize
• CPC Class Codes: C12N 9/18 Carboxylic ester hydrolases (3.1.1)
• CPC Class Codes: C12N 9/2402 hydrolysing O- and S- glycosyl compounds (3.2.1)
• CPC Class Codes: C12N 9/2405 Glucanases
• CPC Class Codes: C12N 9/48 acting on peptide bonds (3.4)
• CPC Class Codes: C12Y 301/01073 Feruloyl esterase (3.1.1.73)
• CPC Class Codes: C12Y 302/01 Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: C12Y 304/00 Hydrolases acting on peptide bonds, i.e. peptidases (3.4)

Abstract

Process for treating crop kernels, comprising the steps of: a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of a feruloyl esterase, wherein step c) is performed before, during or after step b).

Claims

1. A process for treating crop kernels, comprising the steps of:

a) soaking kernels in water to produce soaked kernels;

b) grinding the soaked kernels; and

c) treating the soaked kernels in the presence of an effective amount of a feruloyl esterase;

wherein step c) is performed before, during or after step b).

2. The process of claim 1, further comprising treating the soaked kernels in the presence of a protease.

3. The process of claim 1, further comprising treating the soaked kernels in the presence of an enzyme selected from the group consisting of an endoglucanase, a xylanase, a cellobiohydrolase I, a cellobiohydrolase II, a GH61, or a combination thereof.

4. The process of claim 1, further comprising treating the soaked kernels in the presence of an endoglucanase.

5. The process of claim 1, further comprising treating the soaked kernels in the presence of a xylanase.

6. The process of claim 1, wherein the kernels are soaked in water for about 2-10 hours.

7. The process of claim 1, wherein the soaking is carried out at a temperature between about 40°

C. and about 60°

C.

8. The process of claim 1, wherein the soaking is carried out at acidic pH.

9. The process of claim 1, wherein the soaking is performed in the presence of between 0.01-1%.

10. The process of claim 1, wherein the crop kernels are from corn (maize), rice, barley, sorghum bean, or fruit hulls, or wheat.

11. The process of claim 1, wherein the feruloyl esterase is derived from a strain of the genus Aspergillus, a strain of Chaetomium, a strain of Humicola, a strain of Thielavia, and/or a strain of Penicillium.

12. (canceled)