NUCLEIC ACID CONSTRUCTS AND METHODS OF USE

• US 20160024576A1
• Filed: 05/27/2015
• Published: 01/28/2016
• Est. Priority Date: 04/30/2009
• Status: Active Grant

• Current Assignee: Prognosys Biosciences Inc.
• Original Assignee: Prognosys Biosciences Inc.
• Inventors: CHEE, Mark S.
• Granted Patent: US 9,783,847 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: C12Q 2525/131 incorporating a restriction site
• CPC Class Codes: C12Q 2525/155 incorporating/generating a new priming site
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2539/103 Serial analysis of gene expression [SAGE]
• CPC Class Codes: C12Q 2563/149 Particles, e.g. beads
• CPC Class Codes: C12Q 2563/179 the label being a nucleic acid

Abstract

The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.

Claims

1. A set of nucleic acid constructs, wherein the constructs of the set comprise:

a region of interest (ROI);

at least one unique identifier; and

at least one amplification site;

wherein the set of constructs comprises at least two constructs with the same ROI and different unique identifiers.

3. The set of claim 1, wherein the constructs comprise a single amplification site adjacent to the ROI and the unique identifier.

4. The set of claim 1, wherein the constructs comprise one or more excision sites.

7. The set of claim 1, wherein the constructs are immobilized on a solid support.

8. The set of claim 7, wherein the constructs are immobilized on beads.

2. The set of claim 2, wherein the constructs comprise two or more amplification sites flanking the ROI and the unique identifier.

5. The set of claim 5, wherein the constructs comprise at least two excision sites flanking the ROI.

6. The set of claim 5, wherein the constructs comprise a single excision site adjacent to the ROI.